ArticleJournal of materials science. Materials in medicine2009
Synthesis and characterization of tyramine-based hyaluronan hydrogels.
Article in Journal of materials science. Materials in medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 163 citations in OpenAlex.
- Protocol for preparing tyramine functionalized hyaluronic acid via aqueous DMTMM-mediated conjugation.STAR protocols · 2026Article
- Dynamic microenvironments for bone organoids: spatiotemporal regulation of angiogenic-osteogenic coupling through advanced hydrogel design.Frontiers in bioengineering and biotechnology · 2026Review
- Hyaluronic acid as a versatile building block for the development of biofunctional hydrogels: In vitro models and preclinical innovations.Materials today. Bio · 2025Article
- Synthesis and Engineering of Hyaluronic Acid-Gelatin Hydrogels with Improved Cellular Attachment and Growth.Polymers · 2024Article
- Bioactive Hydrogels Based on Tyramine and Maleimide Functionalized Dextran for Tissue Engineering Applications.Gels (Basel, Switzerland) · 2024Article
- Biomimetic hydrogel scaffolds via enzymatic reaction for cartilage tissue engineering.BMC research notes · 2022Article
- Advanced 3D-Printing Bioinks for Articular Cartilage Repair.International journal of bioprinting · 2022Review
- Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels.Chemical reviews · 2021Review
- Mechanotransducive Biomimetic Systems for Chondrogenic Differentiation In Vitro.International journal of molecular sciences · 2021Review
- Enzymatically crosslinked tyramine-gellan gum hydrogels as drug delivery system for rheumatoid arthritis treatment.Drug delivery and translational research · 2021Article
- Soft Materials by Design: Unconventional Polymer Networks Give Extreme Properties.Chemical reviews · 2021Review
- Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity.Nature communications · 2021Article
- Biodegradable hyaluronan hydrogel coatings on acellular dermis grafts-A potential strategy to improve biologic graft durability in hernia repair application.Journal of biomedical materials research. Part B, Applied biomaterials · 2019Article
- Experimental and Computational Study to Reveal the Potential of Non-Polar Constituents fromMarine drugs · 2019Article
- Tyramine-conjugated alginate hydrogels as a platform for bioactive scaffolds.Journal of biomedical materials research. Part A · 2019Article
- Hyaluronan-Based Grafting Strategies for Liver Stem Cell Therapy and Tracking Methods.Stem cells international · 2019Review
- 3d Tissue Engineered In Vitro Models Of Cancer In Bone.ACS biomaterials science & engineering · 2018Article
- Tyramine functionalization of poly(glycerol sebacate) increases the elasticity of the polymer.Journal of materials chemistry. B · 2017Article
- Enzymatically crosslinked silk-hyaluronic acid hydrogels.Biomaterials · 2017Article
- Photocrosslinked tyramine-substituted hyaluronate hydrogels with tunable mechanical properties improve immediate tissue-hydrogel interfacial strength in articular cartilage.Journal of biomaterials science. Polymer edition · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyaluronan is particularly attractive for tissue engineering and repair because it: (1) is a normal component of the extracellular matrices of most mammalian tissues; (2) contributes to the biological and physical functions of these tissues; and (3) possesses excellent biocompatibility and physiochemical properties. In the present study, we characterize a two-step enzymatic cross-linking chemistry for production of tyramine-based hyaluronan hydrogels using fluorophore-assisted carbohydrate electrophoresis, enzymatic digestion, and spectroscopy including absorbance, fluorescence and (1)H NMR. Substitution on hyaluronan of tyramine and other adducts from unproductive side reactions depends on the molar ratio of tyramine to carbodiimide used during the substitution (step 1) reaction. Results indicate that relatively low tyramine substitution is required to form stable hydrogels, leaving the majority of hyaluronan disaccharides unmodified. Sufficient native HA structure is maintained to allow recognition and binding by b-HABP, a HA binding complex typically found in normal cartilage biology. Hydrogels were formed from tyramine-substituted hyaluronan through a peroxidase-dependent cross-linking (step 2) reaction at hyaluronan concentrations of 2.5 mg/ml and above. Uncross-linked tyramine-substituted hyaluronan was characterized after hyaluronidase SD digestion. Cross-linked hydrogels showed increased resistance to digestion by testicular hyaluronidase and hyaluronidase SD with increasing hyaluronan concentration. Cells directly encapsulated within the hydrogels during hydrogel cross-linking remained metabolically active during 7 days of culture similar to cells cultured in monolayer.
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