ArticlePlant physiology2008
Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 118 citations in OpenAlex.
- Systematic comparison of local approaches for isotopically nonstationary metabolic flux analysis.Frontiers in plant science · 2023Pooled it
- Non-canonical plant metabolism.Nature plants · 2025Review
- A diel multi-tissue genome-scale metabolic model of Vitis vinifera.PLoS computational biology · 2024Article
- SIMPEL: using stable isotopes to elucidate dynamics of context specific metabolism.Communications biology · 2024Article
- Plant Heterotrophic Cultures: No Food, No Growth.Plants (Basel, Switzerland) · 2024Review
- Isotopically non-stationary metabolic flux analysis of heterotrophicFrontiers in plant science · 2022Article
- Manganese toxicity disrupts indole acetic acid homeostasis and suppresses the COScientific reports · 2021Article
- Thermodynamic Genome-Scale Metabolic Modeling of Metallodrug Resistance in Colorectal Cancer.Cancers · 2021Article
- Current Challenges in Plant Systems Biology.Advances in experimental medicine and biology · 2021Article
- A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos.Plant physiology · 2019Article
- Limitations of Deuterium-Labelled Substrates for Quantifying NADPH Metabolism in HeterotrophicMetabolites · 2019Article
- Position-Specific Metabolic Probing and Metagenomics of Microbial Communities Reveal Conserved Central Carbon Metabolic Network Activities at High Temperatures.Frontiers in microbiology · 2019Article
- The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling.Scientific reports · 2018Article
- Modeling the Metabolism ofFrontiers in genetics · 2017Article
- Rapid in situPlant methods · 2017Article
- Metabolic model of central carbon and energy metabolisms of growing Arabidopsis thaliana in relation to sucrose translocation.BMC plant biology · 2016Article
- Reduced mitochondrial malate dehydrogenase activity has a strong effect on photorespiratory metabolism as revealed by 13C labelling.Journal of experimental botany · 2016Article
- Relationships of Leaf Net Photosynthesis, Stomatal Conductance, and Mesophyll Conductance to Primary Metabolism: A Multispecies Meta-Analysis Approach.Plant physiology · 2016Review
- Novel Approach for High-Throughput Metabolic Screening of Whole Plants by Stable Isotopes.Plant physiology · 2016Article
- Flux Balance Analysis of Plant Metabolism: The Effect of Biomass Composition and Model Structure on Model Predictions.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Steady-state labeling experiments with [1-(13)C]Glc were used to measure multiple metabolic fluxes through the pathways of central metabolism in a heterotrophic cell suspension culture of Arabidopsis (Arabidopsis thaliana). The protocol was based on in silico modeling to establish the optimal labeled precursor, validation of the isotopic and metabolic steady state, extensive nuclear magnetic resonance analysis of the redistribution of label into soluble metabolites, starch, and protein, and a comprehensive set of biomass measurements. Following a simple modification of the cell culture procedure, cells were grown at two oxygen concentrations, and flux maps of central metabolism were constructed on the basis of replicated experiments and rigorous statistical analysis. Increased growth rate at the higher O(2) concentration was associated with an increase in fluxes throughout the network, and this was achieved without any significant change in relative fluxes despite differences in the metabolite profile of organic acids, amino acids, and carbohydrates. The balance between biosynthesis and respiration within the tricarboxylic acid cycle was unchanged, with 38% +/- 5% of carbon entering used for biosynthesis under standard O(2) conditions and 33% +/- 2% under elevated O(2). These results add to the emerging picture of the stability of the central metabolic network and its capacity to respond to physiological perturbations with the minimum of rearrangement. The lack of correlation between the change in metabolite profile, which implied significant disruption of the metabolic network following the alteration in the oxygen supply, and the unchanging flux distribution highlights a potential difficulty in the interpretation of metabolomic data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.