ArticlePlant physiology2008
Comparative transcriptomics of Arabidopsis sperm cells.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 188 papers, 2 of them syntheses that pooled it.
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Who cites it
188 citing papers in PubMed, 2 syntheses or guidelines pooled it, 371 citations in OpenAlex.
- Meta-analysis of the expression profiles of the Arabidopsis ESCRT machinery.Plant signaling & behavior · 2011Pooled it
- metaXCMS: second-order analysis of untargeted metabolomics data.Analytical chemistry · 2011Pooled it
- The sperm cell-surface protein GEX3 coordinates with GEX2 and DMP8/9 to mediate sperm-egg adhesion and fusion in Arabidopsis.Plant communications · 2026Article
- Epigenetic Regulation Links Bacterial Signaling to Growth and Reproductive Development in the Green Macroalga Ulva compressa (Chlorophyta).Marine biotechnology (New York, N.Y.) · 2026Article
- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis.The EMBO journal · 2026Article
- Dynamic changes in chromatin structure and transcriptional activity in the generative cells of Lilium longiflorum.Journal of plant research · 2025Article
- Bioimaging insights into structural pathways of cell-to-cell communication within the male (MGU) and female (FGU) germ units of Arabidopsis thaliana.Plant cell reports · 2025Article
- Regulation of cell cycle in plant gametes: when is the right time to divide?Development (Cambridge, England) · 2025Review
- DUO1 Activated Zinc Finger (AtDAZ) protein role in the generative cell body morphogenesis.Plant molecular biology · 2025Article
- The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis.Plant reproduction · 2024Article
- Fifty years of sperm cell isolations: from structural to omic studies.Journal of experimental botany · 2023Article
- Epigenetic Regulation During Plant Development and the Capacity for Epigenetic Memory.Annual review of plant biology · 2023Review
- Cell-type-specific alternative splicing in the Arabidopsis germline.Plant physiology · 2023Article
- Endosperm and Maternal-specific expression of EIN2 in the endosperm affects endosperm cellularization and seed size in Arabidopsis.Genetics · 2023Article
- Removal of the endoplasma membrane upon sperm cell activation after pollen tube discharge.Frontiers in plant science · 2023Article
- The egg cell is preferentially fertilized in Arabidopsis double fertilization.Journal of integrative plant biology · 2022Article
- Epigenetic Dynamics and Regulation of Plant Male Reproduction.International journal of molecular sciences · 2022Review
- Isolation of gametes and zygotes from Setaria viridis.Journal of plant research · 2022Article
- Genome-Wide Identification and Analysis of Ariadne Gene Family Reveal Its Genetic Effects on Agronomic Traits ofInternational journal of molecular sciences · 2022Article
- The poly(A) polymerase PAPS1 mediates pollen maturation by regulating sperm cell differentiation in plants.Plant direct · 2022Article
128 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In flowering plants, the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Understanding the mechanisms and conditions by which male gametes mature and take part in fertilization are crucial goals in the study of plant reproduction. Studies of gene expression in male gametes of maize (Zea mays) and Plumbago and in lily (Lilium longiflorum) generative cells already showed that the previously held view of transcriptionally inert male gametes was not true, but genome-wide studies were lacking. Analyses in the model plant Arabidopsis (Arabidopsis thaliana) were hindered, because no method to isolate sperm cells was available. Here, we used fluorescence-activated cell sorting to isolate sperm cells from Arabidopsis, allowing GeneChip analysis of their transcriptome at a genome-wide level. Comparative analysis of the sperm cell transcriptome with those of representative sporophytic tissues and of pollen showed that sperm has a distinct and diverse transcriptional profile. Functional classifications of genes with enriched expression in sperm cells showed that DNA repair, ubiquitin-mediated proteolysis, and cell cycle progression are overrepresented Gene Ontology categories. Moreover, analysis of the small RNA and DNA methylation pathways suggests that distinct mechanisms might be involved in regulating the epigenetic state of the paternal genome. We identified numerous candidate genes whose involvement in sperm cell development and fertilization can now be directly tested in Arabidopsis. These results provide a roadmap to decipher the role of sperm-expressed proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.