ArticleClinical and vaccine immunology : CVI2008
Use of GRA6-derived synthetic polymorphic peptides in an immunoenzymatic assay to serotype Toxoplasma gondii in human serum samples collected from three continents.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review and meta-analysis of Toxoplasma gondii infection among the Mexican population.Parasites & vectors · 2012Pooled it
- A combination of GRA3, GRA6 and GRA7 peptides offer a useful tool for serotyping type II and IIIFrontiers in cellular and infection microbiology · 2024Article
- Serotyping, a challenging approach forFrontiers in medicine · 2023Review
- Article
- Development of a new serotyping ELISA for Toxoplasma gondii type II, type III and Africa 1 lineages using in silico peptide discovery methods, well categorized feline and human outbreak serum samples.BMC infectious diseases · 2022Article
- Biosensor Based Immunoassay: A New Approach for Serotyping ofNanomaterials (Basel, Switzerland) · 2021Article
- Article
- Serotyping ofFrontiers in cellular and infection microbiology · 2019Article
- Testing New Peptides FromFrontiers in cellular and infection microbiology · 2019Article
- Importance of serological cross-reactivity among Toxoplasma gondii, Hammondia spp., Neospora spp., Sarcocystis spp. and Besnoitia besnoiti.Parasitology · 2017Review
- Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal.Parasitology research · 2017Article
- Diagnosis of toxoplasmosis and typing of Toxoplasma gondii.Parasites & vectors · 2015Review
- Strain hypothesis of Toxoplasma gondii infection on the outcome of human diseases.Acta physiologica (Oxford, England) · 2015Review
- Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphic dense granule protein GRA6.The Journal of experimental medicine · 2014Article
- Genotype analysis of T. gondii strains associated with human infection in Egypt.Parasitology research · 2014Article
- Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.PloS one · 2013Article
- Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.PloS one · 2012Article
- Severe pulmonary toxoplasmosis after allo-SCT in two patients: from Toxoplasma genotyping to clinical management.Bone marrow transplantation · 2010Article
- Selection of polymorphic peptides from GRA6 and GRA7 sequences of Toxoplasma gondii strains to be used in serotyping.Clinical and vaccine immunology : CVI · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serotyping is a simple typing method that consists of an immunoenzymatic assay (enzyme-linked immunosorbent assay [ELISA]) using synthetic polymorphic peptides derived from Toxoplasma gondii antigens. We developed a new ELISA based on GRA6 C-terminal polymorphic peptides. Serum samples from 41 human infections due to 23 archetypal (type I, II, or III) and 18 nonarchetypal strains were selected in order to validate this approach. For 20 out of the 23 archetypal infections, there was a clear correlation between microsatellite genotype and GRA6 serotyping. All infections due to nonarchetypal strains were misclassified as archetypal strain infections. The GRA6 C-terminal peptides from these strains were analyzed to explain this misclassification. A second group of 455 patients with acute and chronic toxoplasmosis due to unknown genotypes from different European, African, and Latin American countries were included in this study, and the strain type predicted by this method. The results suggest that serotyping is a promising method for typing strains, although limitations exist for African and South American strains as a consequence of higher peptide polymorphism. Other peptides from different markers must be studied in order to discriminate archetypal from nonarchetypal strains.
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