ArticleJournal of clinical microbiology2008
Determination of human rotavirus VP6 genogroups I and II by reverse transcription-PCR.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Molecular Characterization of Equine-like G3P[8] Rotavirus Strains Detected in South Korean Children.Viruses · 2025Article
- Rotavirus Infection in Children Suffering from Diarrhea in Patna, Bihar Region, India: A Hospital-Based Study.The American journal of tropical medicine and hygiene · 2025Article
- Rotavirus VP6: involvement in immunogenicity, adjuvant activity, and use as a vector for heterologous peptides, drug delivery, and production of nano-biomaterials.Archives of virology · 2022Review
- The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients.Osong public health and research perspectives · 2021Article
- Current status of rotavirus vaccines.World journal of pediatrics : WJP · 2015Review
- Disease caused by rotavirus infection.The open virology journal · 2014Article
- Genetic diversity of rotavirus genome segment 6 (encoding VP6) in Pretoria, South Africa.SpringerPlus · 2014Article
- Rotavirus genotype distribution during the pre-vaccine period in Bolivia: 2007-2008.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2013Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on nucleotide sequence and phylogenetic analysis of the partial VP6 genes, group A rotaviruses can be mainly differentiated into two genogroups. In this study, a method employing reverse transcription-PCR (RT-PCR) and degenerate primers was established to assign the VP6 genogroup. VP6 genogroup I and genogroup II could be determined according to the sizes of the amplicons: 380 and 780 bp, respectively. The VP6 genogroup of human reference strains of G1 to G4 and G9 types and RotaTeq vaccine strains could be properly assigned by RT-PCR. Eighty rotavirus-positive fecal samples were subjected to enzyme-linked immunosorbent assay (ELISA), RT-PCR, and sequencing of the partial VP6 gene for subgroup and genogroup determination. The results correlated well among these three methods, except for seven samples whose subgroups could not be determined by ELISA. VP6 genogroups of another 150 rotavirus strains recovered between 1981 and 2005 were determined by RT-PCR and sequencing, and the same results were obtained by these two methods. Furthermore, an additional 524 rotavirus-positive fecal samples were tested by RT-PCR, and the VP6 genogroups could be easily determined. The RT-PCR assay developed here provided a reliable and convenient method for assigning the VP6 genogroups of human rotaviruses with a wide range of genetic variation.
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