ArticleDevelopment (Cambridge, England)2008
RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila.
Article in Development (Cambridge, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Different Protein Groups Involved in Transcription Regulation in Development and Housekeeping Genes in Drosophila.Doklady. Biochemistry and biophysics · 2023Article
- Rebelled epigenome: histone H3S10 phosphorylation and H3S10 kinases in cancer biology and therapy.Clinical epigenetics · 2020Review
- H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation.Development (Cambridge, England) · 2017Article
- Digitor/dASCIZ Has Multiple Roles in Drosophila Development.PloS one · 2016Article
- Dosage compensation in Drosophila.Cold Spring Harbor perspectives in biology · 2015Review
- Article
- Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in Drosophila.Nucleic acids research · 2014Article
- Enforcing the pause: transcription factor Sp3 limits productive elongation by RNA polymerase II.Cell cycle (Georgetown, Tex.) · 2013Article
- Transcription factor Sp3 represses expression of p21CIP¹ via inhibition of productive elongation by RNA polymerase II.Molecular and cellular biology · 2013Article
- Evidence against a role for the JIL-1 kinase in H3S28 phosphorylation and 14-3-3 recruitment to active genes in Drosophila.PloS one · 2013Article
- Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promoters.Genome research · 2012Article
- The chromosomal proteins JIL-1 and Z4/Putzig regulate the telomeric chromatin in Drosophila melanogaster.PLoS genetics · 2012Article
- The epigenetic H3S10 phosphorylation mark is required for counteracting heterochromatic spreading and gene silencing in Drosophila melanogaster.Journal of cell science · 2011Article
- Chromatin-associated RNA interference components contribute to transcriptional regulation in Drosophila.Nature · 2011Article
- Global analysis of the relationship between JIL-1 kinase and transcription.PLoS genetics · 2011Article
- 14-3-3 mediates histone cross-talk during transcription elongation in Drosophila.PLoS genetics · 2010Article
- Preparation of Drosophila polytene chromosome squashes for antibody labeling.Journal of visualized experiments : JoVE · 2010Article
- Polytene chromosome squash methods for studying transcription and epigenetic chromatin modification in Drosophila using antibodies.Methods (San Diego, Calif.) · 2009Review
- Article
- Promoter proximal pausing on genes in metazoans.Chromosoma · 2009Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
JIL-1 is the major kinase controlling the phosphorylation state of histone H3S10 at interphase in Drosophila. In this study, we used three different commercially available histone H3S10 phosphorylation antibodies, as well as an acid-free polytene chromosome squash protocol that preserves the antigenicity of the histone H3S10 phospho-epitope, to examine the role of histone H3S10 phosphorylation in transcription under both heat shock and non-heat shock conditions. We show that there is no redistribution or upregulation of JIL-1 or histone H3S10 phosphorylation at transcriptionally active puffs in such polytene squash preparations after heat shock treatment. Furthermore, we provide evidence that heat shock-induced puffs in JIL-1 null mutant backgrounds are strongly labeled by antibody to the elongating form of RNA polymerase II (Pol IIoser2), indicating that Pol IIoser2 is actively involved in heat shock-induced transcription in the absence of histone H3S10 phosphorylation. This is supported by the finding that there is no change in the levels of Pol IIoser2 in JIL-1 null mutant backgrounds compared with wild type. mRNA from the six genes that encode the major heat shock protein in Drosophila, Hsp70, is transcribed at robust levels in JIL-1 null mutants, as directly demonstrated by qRT-PCR. Taken together, these data are inconsistent with the model that Pol II-dependent transcription at active loci requires JIL-1-mediated histone H3S10 phosphorylation, and instead support a model in which transcriptional defects in the absence of histone H3S10 phosphorylation are a result of structural alterations of chromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.