ArticleBMC cell biology2008
Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway.
Article in BMC cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Wasl is crucial to maintain microglial core activities during glioblastoma initiation stages.Glia · 2022Article
- FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.Frontiers in immunology · 2021Article
- Molecular analysis of axonal-intrinsic and glial-associated co-regulation of axon degeneration.Cell death & disease · 2017Article
- WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility.The Journal of cell biology · 2017Article
- In vitro anti-Leishmania activity of tetracyclic iridoids from Morinda lucida, benth.Tropical medicine and health · 2016Article
- Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells.PloS one · 2015Article
- The F-BAR protein PSTPIP1 controls extracellular matrix degradation and filopodia formation in macrophages.Blood · 2014Article
- Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation.The Journal of clinical investigation · 2013Article
- Impact of the carbazole derivative wiskostatin on mechanical stability and dynamics of motile cells.Journal of muscle research and cell motility · 2012Article
- Revolving movement of a dynamic cluster of actin filaments during mitosis.The Journal of cell biology · 2010Article
- Subgroup II PAK-mediated phosphorylation regulates Ran activity during mitosis.The Journal of cell biology · 2010Article
- Physical mechanisms of signal integration by WASP family proteins.Annual review of biochemistry · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCytokinesis is the final step of cell division taking place at the end of mitosis during which the cytoplasmic content and replicated chromosomes of a cell are equally partitioned between the two daughter cells. This process is achieved by the formation and the ingression of an actomyosin contractile ring under the control of equatorial microtubules. The mechanisms of contractile ring formation are not fully understood but involve recruitment of preexisting actin filaments and de novo actin polymerisation.
resultsIn this study, we evaluated the role of the actin nucleation factor, Arp2/3 complex, during cytokinesis. We found that the Arp2/3 complex is recruited late to the cleavage furrow suggesting a potential involvement of Arp2/3 complex during this process. Furthermore, wiskostatin a potent inhibitor of N-WASP activity towards the Arp2/3 complex blocked cytokinesis without affecting mitosis. Nonetheless, this inhibition could not be reproduced using alternative approaches targeting the N-WASP/Arp2/3 complex pathway.
conclusionWe conclude that the wiskostatin induced defective cytokinesis does not occur through the inhibition of the N-WASP/Arp2/3 pathway. Wiskostatin is likely to either directly target other proteins required for cytokinesis progression or alternately wiskostatin bound to N-WASP could affect the activity of other factors involved in cytokinesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.