ArticlePloS one2008
An optimized chloroplast DNA extraction protocol for grasses (Poaceae) proves suitable for whole plastid genome sequencing and SNP detection.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Comprehensive analysis of chloroplast genome evolution in Poaceae: codon usage patterns, selection pressures, and phylogenomic relationships.BMC genomics · 2026Article
- A novel in-situ-process technique constructs whole circular cpDNA library.Plant methods · 2024Article
- Poaceae Chloroplast Genome Sequencing: Great Leap Forward in Recent Ten Years.Current genomics · 2023Review
- Comparative Genomics and Phylogenetic Analysis of the Chloroplast Genomes in Three MedicinalInternational journal of molecular sciences · 2022Article
- Characterizing chloroplast genomes and inferring maternal divergence of the Triticum-Aegilops complex.Scientific reports · 2021Article
- Whole-Genome Sequence of Synthesized Allopolyploids inAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Characteristics analysis of the completePhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Isolation of Intact Chloroplast for Sequencing Plastid Genomes of FivePlants (Basel, Switzerland) · 2019Article
- Developing Chloroplast Genomic Resources from 25Plants (Basel, Switzerland) · 2019Article
- Assembly of chloroplast genomes with long- and short-read data: a comparison of approaches using Eucalyptus pauciflora as a test case.BMC genomics · 2018Article
- NaOH low-salt method for chloroplast isolation and highly pure cpDNA preparation fromApplications in plant sciences · 2018Article
- Nuclear, chloroplast, and mitochondrial data of a US cannabis DNA database.International journal of legal medicine · 2018Article
- Oat evolution revealed in the maternal lineages of 25 Avena species.Scientific reports · 2018Article
- Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing andFrontiers in plant science · 2018Article
- Integral Phylogenomic Approach over Ilex L. Species from Southern South America.Life (Basel, Switzerland) · 2017Article
- Comparative Proteomic and Physiological Analysis Reveals the Variation Mechanisms of Leaf Coloration and Carbon Fixation in a Xantha Mutant of Ginkgo biloba L.International journal of molecular sciences · 2016Article
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- New chloroplast microsatellite markers suitable for assessing genetic diversity of Lolium perenne and other related grass species.Annals of botany · 2012Article
- An improved chloroplast DNA extraction procedure for whole plastid genome sequencing.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundObtaining chloroplast genome sequences is important to increase the knowledge about the fundamental biology of plastids, to understand evolutionary and ecological processes in the evolution of plants, to develop biotechnological applications (e.g. plastid engineering) and to improve the efficiency of breeding schemes. Extraction of pure chloroplast DNA is required for efficient sequencing of chloroplast genomes. Unfortunately, most protocols for extracting chloroplast DNA were developed for eudicots and do not produce sufficiently pure yields for a shotgun sequencing approach of whole plastid genomes from the monocot grasses. METHODOLOGY/PRINCIPAL
findingsWe have developed a simple and inexpensive method to obtain chloroplast DNA from grass species by modifying and extending protocols optimized for the use in eudicots. Many protocols for extracting chloroplast DNA require an ultracentrifugation step to efficiently separate chloroplast DNA from nuclear DNA. The developed method uses two more centrifugation steps than previously reported protocols and does not require an ultracentrifuge. CONCLUSIONS/SIGNIFICANCE: The described method delivered chloroplast DNA of very high quality from two grass species belonging to highly different taxonomic subfamilies within the grass family (Lolium perenne, Pooideae; Miscanthus x giganteus, Panicoideae). The DNA from Lolium perenne was used for whole chloroplast genome sequencing and detection of SNPs. The sequence is publicly available on EMBL/GenBank.
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