ArticlePloS one2008
Reversible disassembly of the actin cytoskeleton improves the survival rate and developmental competence of cryopreserved mouse oocytes.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- From Cytoskeletal Remodeling to Oocyte Quality: The Emerging Role of Mechanics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- How great thou ART: biomechanical properties of oocytes and embryos as indicators of quality in assisted reproductive technologies.Frontiers in cell and developmental biology · 2024Review
- Effect of simulated microgravity conditions of hindlimb unloading on mice hematopoietic and mesenchymal stromal cells.Cell biology international · 2020Article
- Active random forces can drive differential cellular positioning and enhance motor-driven transport.Molecular biology of the cell · 2020Article
- Active diffusion in oocytes nonspecifically centers large objects during prophase I and meiosis I.The Journal of cell biology · 2020Article
- Enhanced cryopreservation of MSCs in microfluidic bioreactor by regulated shear flow.Scientific reports · 2016Article
- Bayesian Inference of Forces Causing Cytoplasmic Streaming in Caenorhabditis elegans Embryos and Mouse Oocytes.PloS one · 2016Article
- Evaluation of the impact of vitrification on the actin cytoskeleton of in vitro matured ovine oocytes by means of Raman microspectroscopy.Journal of assisted reproduction and genetics · 2015Article
- The impact of vitrification on immature oocyte cell cycle and cytoskeletal integrity in a rat model.Journal of assisted reproduction and genetics · 2014Article
- The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells.Cytotechnology · 2014Article
- Model microgravity enhances endothelium differentiation of mesenchymal stem cells.Die Naturwissenschaften · 2013Article
- Freeze-drying of mononuclear cells derived from umbilical cord blood followed by colony formation.PloS one · 2009Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Effective cryopreservation of oocytes is critically needed in many areas of human reproductive medicine and basic science, such as stem cell research. Currently, oocyte cryopreservation has a low success rate. The goal of this study was to understand the mechanisms associated with oocyte cryopreservation through biophysical means using a mouse model. Specifically, we experimentally investigated the biomechanical properties of the ooplasm prior and after cryopreservation as well as the consequences of reversible dismantling of the F-actin network in mouse oocytes prior to freezing. The study was complemented with the evaluation of post-thaw developmental competence of oocytes after in vitro fertilization. Our results show that the freezing-thawing process markedly alters the physiological viscoelastic properties of the actin cytoskeleton. The reversible depolymerization of the F-actin network prior to freezing preserves normal ooplasm viscoelastic properties, results in high post-thaw survival and significantly improves developmental competence. These findings provide new information on the biophysical characteristics of mammalian oocytes, identify a pathophysiological mechanism underlying cryodamage and suggest a novel cryopreservation method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.