ArticlePloS one2008
The N-terminal domain of the Drosophila retinoblastoma protein Rbf1 interacts with ORC and associates with chromatin in an E2F independent manner.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Developmental and Housekeeping Genes: Two Types of Genetic Organization in theInternational journal of molecular sciences · 2024Review
- Direct Regulation of DNA Repair by E2F and RB in Mammals and Plants: Core Function or Convergent Evolution?Cancers · 2021Review
- pRb controls estrogen receptor alpha protein stability and activity.Oncotarget · 2013Article
- Deciphering the retinoblastoma protein phosphorylation code.Trends in biochemical sciences · 2013Review
- Identification of E2F target genes that are rate limiting for dE2F1-dependent cell proliferation.Developmental dynamics : an official publication of the American Association of Anatomists · 2012Article
- Newly identified aspects of tumor suppression by RB.Disease models & mechanisms · 2011Review
- Retinoblastoma protein goes green: the role of RBR in Arabidopsis meiosis.The EMBO journal · 2011Article
- Retinoblastoma protein is essential for early meiotic events in Arabidopsis.The EMBO journal · 2011Article
- Interaction of the retinoblastoma protein with Orc1 and its recruitment to human origins of DNA replication.PloS one · 2010Article
- The RB/E2F pathway and regulation of RNA processing.Biochemical and biophysical research communications · 2009Review
- Sqd interacts with the Drosophila retinoblastoma tumor suppressor Rbf.Biochemical and biophysical research communications · 2009Article
- Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication.Cell cycle (Georgetown, Tex.) · 2009Article
- The origin recognition complex protein family.Genome biology · 2009Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe retinoblastoma (Rb) tumor suppressor protein can function as a DNA replication inhibitor as well as a transcription factor. Regulation of DNA replication may occur through interaction of Rb with the origin recognition complex (ORC). PRINCIPAL
findingsWe characterized the interaction of Drosophila Rb, Rbf1, with ORC. Using expression of proteins in Drosophila S2 cells, we found that an N-terminal Rbf1 fragment (amino acids 1-345) is sufficient for Rbf1 association with ORC but does not bind to dE2F1. We also found that the C-terminal half of Rbf1 (amino acids 345-845) interacts with ORC. We observed that the amino-terminal domain of Rbf1 localizes to chromatin in vivo and associates with chromosomal regions implicated in replication initiation, including colocalization with Orc2 and acetylated histone H4. CONCLUSIONS/SIGNIFICANCE: Our results suggest that Rbf1 can associate with ORC and chromatin through domains independent of the E2F binding site. We infer that Rbf1 may play a role in regulating replication directly through its association with ORC and/or chromatin factors other than E2F. Our data suggest an important role for retinoblastoma family proteins in cell proliferation and tumor suppression through interaction with the replication initiation machinery.
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