ArticleMicrobial cell factories2008
Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000.
Article in Microbial cell factories, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- A gut-derivedmBio · 2025Article
- Whole-Cell Biocatalytic Production of Acetoin with anFoods (Basel, Switzerland) · 2023Article
- Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000.Microbial cell factories · 2017Article
- Fusion protein comprised of the two schistosomal antigens, Sm14 and Sm29, provides significant protection against Schistosoma mansoni in murine infection model.BMC infectious diseases · 2015Article
- IsdC from Staphylococcus lugdunensis induces biofilm formation under low-iron growth conditions.Infection and immunity · 2014Article
- BsaB, a novel adherence factor of group B Streptococcus.Infection and immunity · 2014Article
- Growth phase-dependent proteomes of the Malaysian isolated Lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis.TheScientificWorldJournal · 2014Article
- Regulation and function of pilus island 1 in group B streptococcus.Journal of bacteriology · 2012Article
- Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system.Microbial cell factories · 2011Article
- Lactococcus lactis M4, a potential host for the expression of heterologous proteins.Microbial cell factories · 2011Article
- Immune responses elicited in mice with recombinant Lactococcus lactis expressing F4 fimbrial adhesin FaeG by oral immunization.Veterinary research communications · 2010Article
- The scientific impact of microbial cell factories.Microbial cell factories · 2008Article
- Efficacy of a Lactococcus lactis ΔpyrG vaccine delivery platform expressing chromosomally integrated hly from Listeria monocytogenes.Bioengineered bugsArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundListeria monocytogenes is a well-characterized food-borne pathogen that infects pregnant women and immunocompromised individuals. Listeriolysin O (LLO) is the major virulence factor of the pathogen and is often used as a diagnostic marker for detection of L. monocytogenes. In addition, LLO represents a potent antigen driving T cell-mediated immunity during infection. In the present work, Lactococcus lactis NZ9000 was used as an expression host to hyper-produce LLO under inducible conditions using the NICE (NIsin Controlled Expression) system. We created a modified pNZ8048 vector encoding a six-His-tagged LLO downstream of the strong inducible PnisA promoter.
resultsThe constructed vector (pNZPnisA:CYTO-LLO) was expressed in L. lactis NZ9000 and was best induced at mid-log phase with 0.2% v/v nisin for 4 h statically at 30 degrees C. Purification of the His-tagged LLO was accomplished by Ni-NTA affinity chromatography and functionality was confirmed through haemolytic assays. Total LLO yield (measured as total protein content) was 4.43-5.9 mg per litre culture and the haemolytic activity was still detectable after 8 months of storage at 4 degrees C.
conclusionThe LLO production method described in this work provides an approach to efficient LLO production in the Gram-positive Lactococcus bacterium to yield a significant source of the protein for research and diagnostic applications. Expression of LLO in L. lactis has a number of benefits over E. coli which may facilitate both in vivo and in vitro applications of this system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.