ArticleThe Journal of cell biology2008
Repulsion by Slit and Roundabout prevents Shotgun/E-cadherin-mediated cell adhesion during Drosophila heart tube lumen formation.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 113 citations in OpenAlex.
- The role of geometry in morphogenesis.Development (Cambridge, England) · 2026Review
- Article
- In Vivo Models of Cardiovascular Disease:Biomedicines · 2025Review
- Focal adhesion in the tumour metastasis: from molecular mechanisms to therapeutic targets.Biomarker research · 2025Review
- The Mechanics of Building Functional Organs.Cold Spring Harbor perspectives in biology · 2025Review
- The dynamics of tubulogenesis in development and disease.Development (Cambridge, England) · 2025Review
- TheeLife · 2025Article
- ThebioRxiv : the preprint server for biology · 2024Article
- Genome-Wide Association Study IdentifiesJournal of clinical oncology : official journal of the American Society of Clinical Oncology · 2023Article
- Expanding the phenome and variome of the ROBO-SLIT pathway in congenital heart defects: toward improving the genetic testing yield of CHD.Journal of translational medicine · 2023Article
- Slit-Robo signalling establishes a Sphingosine-1-phosphate gradient to polarise fin mesenchyme.EMBO reports · 2022Article
- Slit/Robo Signaling Regulates Multiple Stages of the Development of theFrontiers in cell and developmental biology · 2021Article
- Extracellular matrix dynamics in tubulogenesis.Cellular signalling · 2020Review
- Cbl-Associated Protein CAP contributes to correct formation and robust function of the Drosophila heart tube.PloS one · 2020Article
- Making Connections: Guidance Cues and Receptors at Nonneural Cell-Cell Junctions.Cold Spring Harbor perspectives in biology · 2018Review
- Insane in the apical membrane: Trafficking events mediating apicobasal epithelial polarity during tube morphogenesis.Traffic (Copenhagen, Denmark) · 2018Review
- Slit-Robo signalling in heart development.Cardiovascular research · 2018Review
- Slit3 regulates migration of endothelial progenitor cells by activation of the RhoA/Rho kinase pathway.International journal of clinical and experimental pathology · 2018Article
- Conserved signaling mechanisms in Drosophila heart development.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Review
- Dissecting the Role of the Extracellular Matrix in Heart Disease: Lessons from the Drosophila Genetic Model.Veterinary sciences · 2017Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
During Drosophila melanogaster heart development, a lumen forms between apical surfaces of contralateral cardioblasts (CBs). We show that Slit and its receptor Roundabout (Robo) are required at CB apical domains for lumen formation. Mislocalization of Slit outside the apical domain causes ectopic lumen formation and the mislocalization of cell junction proteins, E-cadherin (E-Cad) and Enabled, without disrupting overall CB cell polarity. Ectopic lumen formation is suppressed in robo mutants, which indicates robo's requirement for this process. Genetic evidence suggests that Robo and Shotgun (Shg)/E-Cad function together in modulating CB adhesion. robo and shg/E-Cad transheterozygotes have lumen defects. In robo loss-of-function or shg/E-Cad gain-of-function embryos, lumen formation is blocked because of inappropriate CB adhesion and an accumulation of E-Cad at the apical membrane. In contrast, shg/E-Cad loss-of-function or robo gain-of-function blocks lumen formation due to a loss of CB adhesion. Our data show that Slit and Robo pathways function in lumen formation as a repulsive signal to antagonize E-Cad-mediated cell adhesion.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.