ArticleInternational journal of legal medicine2009
Automatic detection of spermatozoa for laser capture microdissection.
Article in International journal of legal medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Spitz: a YOLO-based deep learning system for automated spermatozoa detection in forensic genetic analysis.International journal of legal medicine · 2026Article
- Evaluating novel and conventional cell-separation techniques for sexual assault investigations.Journal of forensic sciences · 2025Article
- Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases.International journal of legal medicine · 2024Review
- Single cell genomics applications in forensic science: Current state and future directions.iScience · 2023Review
- Improved resolution of mixed STR profiles using a fully automated differential cell lysis/DNA extraction method.Forensic sciences research · 2020Article
- Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review).International journal of legal medicine · 2018Review
- Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process.International journal of legal medicine · 2017Article
- Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis.Biomicrofluidics · 2015Article
- The role of histopathology in forensic practice: an overview.Forensic science, medicine, and pathology · 2014Review
- Article
- Forensic DNA research: keeping it real.International journal of legal medicine · 2012Article
- Immunohistochemical staining of human sperm cells in smears from sexual assault cases.International journal of legal medicine · 2011Article
- New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks.Croatian medical journal · 2011Article
- Article
- Article
- Forensic trace DNA: a review.Investigative genetics · 2010Article
- Laser capture microdissection in forensic research: a review.International journal of legal medicine · 2010Review
- Laser capture microdissection in the tissue biorepository.Journal of biomolecular techniques : JBT · 2010Review
- Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures.International journal of legal medicine · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In sexual assault crimes, differential extraction of spermatozoa from vaginal swab smears is often ineffective, especially when only a few spermatozoa are present in an overwhelming amount of epithelial cells. Laser capture microdissection (LCM) enables the precise separation of spermatozoa and epithelial cells. However, standard sperm-staining techniques are non-specific and rely on sperm morphology for identification. Moreover, manual screening of the microscope slides is time-consuming and labor-intensive. Here, we describe an automated screening method to detect spermatozoa stained with Sperm HY-LITER. Different ratios of spermatozoa and epithelial cells were used to assess the automatic detection method. In addition, real postcoital samples were also screened. Detected spermatozoa were isolated using LCM and DNA analysis was performed. Robust DNA profiles without allelic dropout could be obtained from as little as 30 spermatozoa recovered from postcoital samples, showing that the staining had no significant influence on DNA recovery.
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