ArticleAnalytical and bioanalytical chemistry2008
Quantitative evaluation of oligonucleotide surface concentrations using polymerization-based amplification.
Article in Analytical and bioanalytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Comparison of eosin and fluorescein conjugates for the photoinitiation of cell-compatible polymer coatings.PloS one · 2018Article
- Protective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation.ACS applied materials & interfaces · 2015Article
- Characterization of molecular transport in ultrathin hydrogel coatings for cellular immunoprotection.Biomacromolecules · 2015Article
- Article
- Glucose oxidase-mediated polymerization as a platform for dual-mode signal amplification and biodetection.Biotechnology and bioengineering · 2011Article
- Sensitive immunofluorescent staining of cells via generation of fluorescent nanoscale polymer films in response to biorecognition.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Article
- Formation of three-dimensional hydrogel multilayers using enzyme-mediated redox chain initiation.ACS applied materials & interfaces · 2010Article
- Development of fluorescent polymerization-based signal amplification for sensitive and non-enzymatic biodetection in antibody microarrays.Acta biomaterialia · 2010Article
- Mechanism of Cyclic Dye Regeneration During Eosin-Sensitized Photoinitiation in the Presence of Polymerization Inhibitors.Journal of polymer science. Part A, Polymer chemistry · 2009Article
- Polymerization Behavior and Polymer Properties of Eosin-Mediated Surface Modification Reactions.Polymer · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Quantitative evaluation of minimal polynucleotide concentrations has become a critical analysis among a myriad of applications found in molecular diagnostic technology. Development of high-throughput, nonenzymatic assays that are sensitive, quantitative and yet feasible for point-of-care testing are thus beneficial for routine implementation. Here, we develop a nonenzymatic method for quantifying surface concentrations of labeled DNA targets by coupling regulated amounts of polymer growth to complementary biomolecular binding on array-based biochips. Polymer film thickness measurements in the 20-220 nm range vary logarithmically with labeled DNA surface concentrations over two orders of magnitude with a lower limit of quantitation at 60 molecules/microm(2) (approximately 10(6) target molecules). In an effort to develop this amplification method towards compatibility with fluorescence-based methods of characterization, incorporation of fluorescent nanoparticles into the polymer films is also evaluated. The resulting gains in fluorescent signal enable quantification using detection instrumentation amenable to point-of-care settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.