ArticleJournal of bacteriology2008
Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp. strain PCC 7120.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 132 citations in OpenAlex.
- Cyanotoxins and Other Bioactive Compounds from the Pasteur Cultures of Cyanobacteria (PCC).Toxins · 2023Review
- Terpene biosynthesis in marine sponge animals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Versatile Applications of Cyanobacteria in Biotechnology.Microorganisms · 2022Review
- Cytochrome P450s in algae: Bioactive natural product biosynthesis and light-driven bioproduction.Acta pharmaceutica Sinica. B · 2022Review
- Identification and Characterization of Bifunctional Drimenol Synthases of Marine Bacterial Origin.ACS chemical biology · 2022Article
- Alternative metabolic pathways and strategies to high-titre terpenoid production inNatural product reports · 2022Review
- Protein Engineering of a Germacrene A Synthase FromFrontiers in plant science · 2022Article
- Bioactive Metabolites Produced by Cyanobacteria for Growth Adaptation and Their Pharmacological Properties.Biology · 2021Review
- Comparing Sediment Microbiomes in Contaminated and Pristine Wetlands along the Coast of Yucatan.Microorganisms · 2021Article
- Improved production of germacrene A, a direct precursor of ß-elemene, in engineered Saccharomyces cerevisiae by expressing a cyanobacterial germacrene A synthase.Microbial cell factories · 2021Article
- Germacrene A-A Central Intermediate in Sesquiterpene Biosynthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Review
- Two Diterpene Synthases from Chryseobacterium: Chryseodiene Synthase and Wanjudiene Synthase.Angewandte Chemie (International ed. in English) · 2020Article
- Developmentally regulated volatiles geosmin and 2-methylisoborneol attract a soil arthropod to Streptomyces bacteria promoting spore dispersal.Nature microbiology · 2020Article
- A novel cyanobacterial geosmin producer, revising GeoA distribution and dispersion patterns in Bacteria.Scientific reports · 2020Article
- Comprehensive Analyses of Cytochrome P450 Monooxygenases and Secondary Metabolite Biosynthetic Gene Clusters inInternational journal of molecular sciences · 2020Article
- Exploring novel bacterial terpene synthases.PloS one · 2020Article
- Transcriptome Analysis of Oleoresin-Producing TreeFrontiers in plant science · 2018Article
- Metabolic engineering of Saccharomyces cerevisiae for production of germacrene A, a precursor of beta-elemene.Journal of industrial microbiology & biotechnology · 2017Article
- Article
- Mechanistic investigations on six bacterial terpene cyclases.Beilstein journal of organic chemistry · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are a rich source of natural products and are known to produce terpenoids. These bacteria are the major source of the musty-smelling terpenes geosmin and 2-methylisoborneol, which are found in many natural water supplies; however, no terpene synthases have been characterized from these organisms to date. Here, we describe the characterization of three sesquiterpene synthases identified in Nostoc sp. strain PCC 7120 (terpene synthase NS1) and Nostoc punctiforme PCC 73102 (terpene synthases NP1 and NP2). The second terpene synthase in N. punctiforme (NP2) is homologous to fusion-type sesquiterpene synthases from Streptomyces spp. shown to produce geosmin via an intermediate germacradienol. The enzymes were functionally expressed in Escherichia coli, and their terpene products were structurally identified as germacrene A (from NS1), the eudesmadiene 8a-epi-alpha-selinene (from NP1), and germacradienol (from NP2). The product of NP1, 8a-epi-alpha-selinene, so far has been isolated only from termites, in which it functions as a defense compound. Terpene synthases NP1 and NS1 are part of an apparent minicluster that includes a P450 and a putative hybrid two-component protein located downstream of the terpene synthases. Coexpression of P450 genes with their adjacent located terpene synthase genes in E. coli demonstrates that the P450 from Nostoc sp. can be functionally expressed in E. coli when coexpressed with a ferredoxin gene and a ferredoxin reductase gene from Nostoc and that the enzyme oxygenates the NS1 terpene product germacrene A. This represents to the best of our knowledge the first example of functional expression of a cyanobacterial P450 in E. coli.
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