ArticleProceedings of the National Academy of Sciences of the United States of America2008
Poly(A) leader of eukaryotic mRNA bypasses the dependence of translation on initiation factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- 40S ribosomal subunits move along the 5' UTR through an eIF4A-independent mechanism in higher eukaryotes.bioRxiv : the preprint server for biology · 2026Article
- 40S ribosomal subunits scan mRNA for the start codon by one-dimensional diffusion.RNA (New York, N.Y.) · 2025Article
- Distinct non-canonical translation initiation modes arise for specific host and viral mRNAs during poxvirus-induced shutoff.Nature microbiology · 2025Article
- Ribosome customization and functional diversification among P-stalk proteins regulate late poxvirus protein synthesis.Cell reports · 2025Article
- Comprehensive translational profiling and STE AI uncover rapid control of protein biosynthesis during cell stress.Nucleic acids research · 2024Article
- Characterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model.Microbial cell factories · 2024Article
- Advances in Understanding the Mechanism of Cap-Independent Cucurbit Aphid-Borne Yellows Virus Protein Synthesis.International journal of molecular sciences · 2023Article
- Terminal hairpins improve protein expression in IRES-initiated mRNA in the absence of a cap and polyadenylated tail.Gene therapy · 2023Article
- Article
- Distance-dependent inhibition of translation initiation by downstream out-of-frame AUGs is consistent with a Brownian ratchet process of ribosome scanning.Genome biology · 2022Article
- Ribosomes in poxvirus infection.Current opinion in virology · 2022Review
- RACK1 Regulates Poxvirus Protein Synthesis Independently of Its Role in Ribosome-Based Stress Signaling.Journal of virology · 2022Article
- Tandem repeats ubiquitously flank and contribute to translation initiation sites.BMC genomic data · 2022Article
- Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics.Nature communications · 2022Article
- PABP1 Drives the Selective Translation of Influenza A Virus mRNA.Journal of molecular biology · 2022Article
- Negative charge in the RACK1 loop broadens the translational capacity of the human ribosome.Cell reports · 2021Article
- Non-Canonical Translation Initiation Mechanisms Employed by Eukaryotic Viral mRNAs.Biochemistry. Biokhimiia · 2021Review
- ATP-Independent Initiation during Cap-Independent Translation of mInternational journal of molecular sciences · 2021Article
- Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics.bioRxiv : the preprint server for biology · 2021Article
- Making ends meet: New functions of mRNA secondary structure.Wiley interdisciplinary reviews. RNA · 2021Review
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic mRNAs in which a poly(A) sequence precedes the initiation codon are known to exhibit a significantly enhanced cap-independent translation, both in vivo and in cell-free translation systems. Consistent with high expression levels of poxviral mRNAs, they contain poly(A) sequences at their 5' ends, immediately before the initiation AUG codon. Here we show that poly(A) as a leader sequence in mRNA constructs promotes the recruitment of the 40S ribosomal subunits and the efficient formation of initiation complexes at cognate AUG initiation codons in the absence of two essential translation initiation factors, eIF3 and eIF4F. These factors are known to be indispensable for the cap-dependent (and ATP-dependent) mechanism of translation initiation but are shown here to be not required if an mRNA contains a 5'-proximal poly(A). Thus, the presence of a pre-AUG poly(A) sequence results in an alternative mechanism of translation initiation. It involves the binding of initiating 40S ribosomal subunits within the 5' UTR and their phaseless, ATP-independent, diffusional movement ("phaseless wandering") along the leader sequence, with subsequent recognition of the initiation (AUG) codon.
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