ArticleBiophysical journal2008
Experimental verification of the behavioral foundation of bacterial transport parameters using microfluidics.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 109 citations in OpenAlex.
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- Optimal Cell Length for Exploration and Exploitation in Chemotactic Planktonic Bacteria.Environmental microbiology · 2024Article
- Surface conversion of the dynamics of bacteria escaping chemorepellents.The European physical journal. E, Soft matter · 2024Article
- Individual bacterial cells can use spatial sensing of chemical gradients to direct chemotaxis on surfaces.Nature microbiology · 2024Article
- Microfluidic approaches in microbial ecology.Lab on a chip · 2024Review
- The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSEnvironmental microbiology · 2024Article
- Suicidal chemotaxis in bacteria.Nature communications · 2022Article
- Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation.Microbial biotechnology · 2022Review
- Habitat geometry in artificial microstructure affects bacterial and fungal growth, interactions, and substrate degradation.Communications biology · 2021Article
- Haloarchaea swim slowly for optimal chemotactic efficiency in low nutrient environments.Nature communications · 2020Article
- Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel.Archives of microbiology · 2019Article
- Cooperation and spatial self-organization determine rate and efficiency of particulate organic matter degradation in marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Bacterial chemotaxis in a microfluidic T-maze reveals strong phenotypic heterogeneity in chemotactic sensitivity.Nature communications · 2019Article
- Microfluidics with fluid walls.Nature communications · 2017Review
- A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities.Nature microbiology · 2017Article
- Propulsion and Chemotaxis in Bacteria-Driven Microswimmers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Article
- Chemotaxis of bio-hybrid multiple bacteria-driven microswimmers.Scientific reports · 2016Article
- Speed-dependent chemotactic precision in marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Enhancement of Swimming Speed Leads to a More-Efficient Chemotactic Response to Repellent.Applied and environmental microbiology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We present novel microfluidic experiments to quantify population-scale transport parameters (chemotactic sensitivity chi(0) and random motility mu) of a population of bacteria. Previously, transport parameters have been derived theoretically from single-cell swimming behavior using probabilistic models, yet the mechanistic foundations of this upscaling process have not been verified experimentally. We designed a microfluidic capillary assay to generate and accurately measure gradients of chemoattractant (alpha-methylaspartate) while simultaneously capturing the swimming trajectories of individual Escherichia coli bacteria using videomicroscopy and cell tracking. By measuring swimming speed and bias in the swimming direction of single cells for a range of chemoattractant concentrations and concentration gradients, we directly computed the chemotactic velocity VC and the associated chemotactic sensitivity chi(0). We then show how mu can also be readily determined using microfluidics but that a population-scale microfluidic approach is experimentally more convenient than a single-cell analysis in this case. Measured values of both chi(0) [(12.4 +/- 2.0) x 10(-4) cm(2) s(-1)] and mu [(3.3 +/- 0.8) x 10(-6) cm(2) s(-1)] are comparable to literature results. This microscale approach to bacterial chemotaxis lends experimental support to theoretical derivations of population-scale transport parameters from single-cell behavior. Furthermore, this study shows that microfluidic platforms can go beyond traditional chemotaxis assays and enable the quantification of bacterial transport parameters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.