ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2008
Construction of signature-tagged mutant library in Mesorhizobium loti as a powerful tool for functional genomics.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 43 citations in OpenAlex.
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- IMA peptides regulate root nodulation and nitrogen homeostasis by providing iron according to internal nitrogen status.Nature communications · 2024Article
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- Evaluation of qPCR to Detect Shifts in Population Composition of the Rhizobial SymbiontBiology · 2023Article
- Reactive Sulfur Species Produced by Cystathionine γ-lyase Function in the Establishment of Mesorhizobium loti-Lotus japonicus Symbiosis.Microbes and environments · 2023Article
- Exploring the onset of BEnvironmental microbiology · 2022Article
- Lineage-Specific Rewiring of Core Pathways Predating Innovation of Legume Nodules Shapes Symbiotic Efficiency.mSystems · 2021Article
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- The rhizobial autotransporter determines the symbiotic nitrogen fixation activity ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Bacterial protein meta-interactomes predict cross-species interactions and protein function.BMC bioinformatics · 2017Article
- Lotus japonicus alters in planta fitness of Mesorhizobium loti dependent on symbiotic nitrogen fixation.PloS one · 2017Article
- Whole-Genome Sequence of the Nitrogen-Fixing Symbiotic Rhizobium Mesorhizobium loti Strain TONO.Genome announcements · 2016Article
- Rhizobial gibberellin negatively regulates host nodule number.Scientific reports · 2016Article
- Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies.BMC genomics · 2015Article
- The National BioResource Project (NBRP) Lotus and Glycine in Japan.Breeding science · 2012Article
- Mining high-throughput experimental data to link gene and function.Trends in biotechnology · 2011Review
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobia are nitrogen-fixing soil bacteria that establish endosymbiosis with some leguminous plants. The completion of several rhizobial genome sequences provides opportunities for genome-wide functional studies of the physiological roles of many rhizobial genes. In order to carry out genome-wide phenotypic screenings, we have constructed a large mutant library of the nitrogen-fixing symbiotic bacterium, Mesorhizobium loti, by transposon mutagenesis. Transposon insertion mutants were generated using the signature-tagged mutagenesis (STM) technique and a total of 29,330 independent mutants were obtained. Along with the collection of transposon mutants, we have determined the transposon insertion sites for 7892 clones, and confirmed insertions in 3680 non-redundant M. loti genes (50.5% of the total number of M. loti genes). Transposon insertions were randomly distributed throughout the M. loti genome without any bias toward G+C contents of insertion target sites and transposon plasmids used for the mutagenesis. We also show the utility of STM mutants by examining the specificity of signature tags and test screenings for growth- and nodulation-deficient mutants. This defined mutant library allows for genome-wide forward- and reverse-genetic functional studies of M. loti and will serve as an invaluable resource for researchers to further our understanding of rhizobial biology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.