ArticleMolecular cell2008
siRNA-mediated heterochromatin establishment requires HP1 and is associated with antisense transcription.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed, 104 citations in OpenAlex.
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- Structural insights into the binding mechanism of Clr4 methyltransferase to H3K9 methylated nucleosome.Scientific reports · 2024Article
- Spt5 C-terminal repeat domain phosphorylation and length negatively regulate heterochromatin through distinct mechanisms.PLoS genetics · 2023Article
- Review
- Investigating Mitotic Inheritance of Histone Modifications Using Tethering Strategies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Defining the consequences of endogenous genetic variation within a novel family of Schizosaccharomyces pombe heterochromatin nucleating sequences.G3 (Bethesda, Md.) · 2021Article
- An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing.PLoS genetics · 2021Article
- Nuclear Envelope Proteins Modulating the Heterochromatin Formation and Functions in Fission Yeast.Cells · 2020Review
- Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.Nucleic acids research · 2020Article
- Transgenerational epigenetic inheritance: from phenomena to molecular mechanisms.Current opinion in neurobiology · 2019Review
- Vigilin protein Vgl1 is required for heterochromatin-mediated gene silencing inThe Journal of biological chemistry · 2019Article
- Article
- Ten principles of heterochromatin formation and function.Nature reviews. Molecular cell biology · 2018Review
- Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.Molecular cell · 2017Article
- Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi.Nature communications · 2017Article
- The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts.Genes & development · 2016Article
- Two sets of RNAi components are required for heterochromatin formation in trans triggered by truncated transgenes.Nucleic acids research · 2016Article
- Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex.Cell reports · 2016Article
- Transcription of tandemly repetitive DNA: functional roles.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heterochromatic gene silencing at the pericentromeric DNA repeats in fission yeast requires the RNA interference (RNAi) machinery. The RNA-induced transcriptional silencing (RITS) complex mediates histone H3 lysine 9 (H3K9) methylation and recruits the RNA-dependent RNA polymerase complex (RDRC) to promote double-stranded RNA (dsRNA) synthesis and siRNA generation. Here we show that ectopic expression of a long hairpin RNA bypasses the requirement for chromatin-dependent steps in siRNA generation. The ability of hairpin-produced siRNAs to silence homologous sequences in trans is subject to local chromatin structure, requires HP1, and correlates with antisense transcription at the target locus. Furthermore, although hairpin siRNAs can be produced in the absence of RDRC, trans-silencing of reporter genes by hairpin-produced siRNAs is completely dependent on the dsRNA synthesis activity of RDRC. These results provide insights into the regulation of siRNA action and reveal roles for cis-dsRNA synthesis and HP1 in siRNA-mediated heterochromatin assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.