ArticleExperimental biology and medicine (Maywood, N.J.)2008
Expanded CD34+ human umbilical cord blood cells generate multiple lymphohematopoietic lineages in NOD-scid IL2rgamma(null) mice.
Article in Experimental biology and medicine (Maywood, N.J.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
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- An Improved Patient-Derived Xenograft Humanized Mouse Model for Evaluation of Lung Cancer Immune Responses.Cancer immunology research · 2019Article
- Development of Onchocerca volvulus in humanized NSG mice and detection of parasite biomarkers in urine and serum.PLoS neglected tropical diseases · 2018Article
- Methylprednisolone acetate induces, and Δ7-dafachronic acid suppresses,Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Replacing mouse BAFF with human BAFF does not improve B-cell maturation in hematopoietic humanized mice.Blood advances · 2017Article
- Interleukins 7 and 15 Maintain Human T Cell Proliferative Capacity through STAT5 Signaling.PloS one · 2016Article
- Traumatic spinal cord injury in mice with human immune systems.Experimental neurology · 2015Article
- A novel, long-lived, and highly engraftable immunodeficient mouse model of mucopolysaccharidosis type I.Molecular therapy. Methods & clinical development · 2015Article
- Stem cell origin of myelodysplastic syndromes.Oncogene · 2014Review
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- Engineering humanized mice for improved hematopoietic reconstitution.Cellular & molecular immunology · 2012Review
- Advancing animal models of human type 1 diabetes by engraftment of functional human tissues in immunodeficient mice.Cold Spring Harbor perspectives in medicine · 2012Review
- Article
- A Kaposi's sarcoma-associated herpesvirus-encoded ortholog of microRNA miR-155 induces human splenic B-cell expansion in NOD/LtSz-scid IL2Rγnull mice.Journal of virology · 2011Article
- Mesenchymal stem cells secreting angiopoietin-like-5 support efficient expansion of human hematopoietic stem cells without compromising their repopulating potential.Stem cells and development · 2011Article
- Generation of hematopoietic humanized mice in the newborn BALB/c-Rag2null Il2rγnull mouse model: a multivariable optimization approach.Clinical immunology (Orlando, Fla.) · 2011Article
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- Establishment of a xenograft model of human myelodysplastic syndromes.Haematologica · 2011Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Umbilical cord blood (UCB) is increasingly being used for human hematopoietic stem cell (HSC) transplantation in children but often requires pooling multiple cords to obtain sufficient numbers for transplantation in adults. To overcome this limitation, we have used an ex vivo two-week culture system to expand the number of hematopoietic CD34(+) cells in cord blood. To assess the in vivo function of these expanded CD34(+) cells, cultured human UCB containing 1 x 10(6) CD34(+) cells were transplanted into conditioned NOD-scid IL2rgamma(null) mice. The expanded CD34(+) cells displayed short- and long-term repopulating cell activity. The cultured human cells differentiated into myeloid, B-lymphoid, and erythroid lineages, but not T lymphocytes. Administration of human recombinant TNFalpha to recipient mice immediately prior to transplantation promoted human thymocyte and T-cell development. These T cells proliferated vigorously in response to TCR cross-linking by anti-CD3 antibody. Engrafted TNFalpha-treated mice generated antibodies in response to T-dependent and T-independent immunization, which was enhanced when mice were co-treated with the B cell cytokine BLyS. Ex vivo expanded CD34(+) human UCB cells have the capacity to generate multiple hematopoietic lineages and a functional human immune system upon transplantation into TNFalpha-treated NOD-scid IL2rgamma(null) mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.