ArticleExperimental cell research2008
Engineering amount of cell-cell contact demonstrates biphasic proliferative regulation through RhoA and the actin cytoskeleton.
Article in Experimental cell research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 63 citations in OpenAlex.
- The Plasma Membrane and Mechanoregulation in Cells.ACS omega · 2024Review
- Adhesion strength between cells regulate nonmonotonic growth by a biomechanical feedback mechanism.Biophysical journal · 2022Article
- Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control.ACS applied materials & interfaces · 2021Article
- Harnessing Mechanobiology for Tissue Engineering.Developmental cell · 2021Review
- A Simple Method for Fabrication of Microstructures Using a PDMS Stamp.Micromachines · 2016Article
- Bridging the Gap: From 2D Cell Culture to 3D Microengineered Extracellular Matrices.Advanced healthcare materials · 2015Review
- CDH2 and CDH11 act as regulators of stem cell fate decisions.Stem cell research · 2015Review
- Engineered culture models for studies of tumor-microenvironment interactions.Annual review of biomedical engineering · 2013Review
- Deciphering the combinatorial roles of geometric, mechanical, and adhesion cues in regulation of cell spreading.PloS one · 2013Article
- Heterotypic cell pair co-culturing on patterned microarrays.Lab on a chip · 2012Article
- Endothelial cell micropatterning: methods, effects, and applications.Annals of biomedical engineering · 2011Review
- Differential migration and proliferation of geometrical ensembles of cell clusters.Experimental cell research · 2011Article
- The generation of biomolecular patterns in highly porous collagen-GAG scaffolds using direct photolithography.Biomaterials · 2011Article
- Engineering extracellular matrix through nanotechnology.Journal of the Royal Society, Interface · 2010Review
- A pathogenic relationship between a regulator of the actin cytoskeleton and serum response factor.Genetics · 2010Article
- SWI/SNF chromatin remodeling enzyme ATPases promote cell proliferation in normal mammary epithelial cells.Journal of cellular physiology · 2010Article
- Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrin-linked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes.Arthritis research & therapy · 2010Article
- Tunable interplay between epidermal growth factor and cell-cell contact governs the spatial dynamics of epithelial growth.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Endothelial cell-cell contact via VE-cadherin plays an important role in regulating numerous cell functions, including proliferation. However, using different experimental approaches to manipulate cell-cell contact, investigators have observed both inhibition and stimulation of proliferation depending on the adhesive context. In this study, we used micropatterned wells combined with active positioning of cells by dielectrophoresis in order to investigate whether the number of contacting neighbors affected the proliferative response. Varying cell-cell contact resulted in a biphasic effect on proliferation; one contacting neighbor increased proliferation, while two or more neighboring cells partially inhibited this increase. We also observed that cell-cell contact increased the formation of actin stress fibers, and that expression of dominant negative RhoA (RhoN19) blocked the contact-mediated increase in stress fibers and proliferation. Furthermore, examination of heterotypic pairs of untreated cells in contact with RhoN19-expressing cells revealed that intracellular, but not intercellular, tension is required for the contact-mediated stimulation of proliferation. Moreover, engagement of VE-cadherin with cadherin-coated beads was sufficient to stimulate proliferation in the absence of actual cell-cell contact. In all, these results demonstrate that cell-cell contact signals through VE-cadherin, RhoA, and intracellular tension in the actin cytoskeleton to regulate proliferation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.