ArticleBMC cancer2008
Conjugated linoleic acid induces apoptosis through estrogen receptor alpha in human breast tissue.
Article in BMC cancer, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 53 citations in OpenAlex.
- Insights into the gut microbiome-metabolite dynamics in breast cancer.Gut microbes reports · 2025Review
- Modulating Microbiota as a New Strategy for Breast Cancer Prevention and Treatment.Microorganisms · 2022Review
- Heat Stress Altered the Vaginal Microbiome and Metabolome in Rabbits.Frontiers in microbiology · 2022Article
- Dietary intake of trans fatty acids and breast cancer risk in 9 European countries.BMC medicine · 2021Article
- Understanding doxorubicin associated calcium remodeling during triple-negative breast cancer treatment: anExploration of targeted anti-tumor therapy · 2021Article
- Effects of Dietary Fatty Acids on Bovine Oocyte Competence and Granulosa Cells.Frontiers in endocrinology · 2020Article
- The effects of conjugated linoleic acids on breast cancer: A systematic review.Advanced biomedical research · 2016Review
- Article
- A lack of ovarian function increases neuroinflammation in aged mice.Endocrinology · 2012Article
- Growth inhibition and apoptotic effect of alpha-eleostearic acid on human breast cancer cells.Journal of natural medicines · 2012Article
- The janus face of lipids in human breast cancer: how polyunsaturated Fatty acids affect tumor cell hallmarks.International journal of breast cancer · 2012Article
- In Vitro Ultramorphological Assessment of Apoptosis on CEMss Induced by Linoleic Acid-Rich Fraction from Typhonium flagelliforme Tuber.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Influence of diet enriched with conjugated linoleic acids on their distribution in tissues of rats with DMBA induced tumors.Lipids in health and disease · 2010Article
- Lipophilic compound-mediated gene expression and implication for intervention in reactive oxygen species (ROS)-related diseases: mini-review.Nutrients · 2010Review
- Anthocyanins in black raspberries prevent esophageal tumors in rats.Cancer prevention research (Philadelphia, Pa.) · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundConjugated linoleic acid (CLA), a naturally occurring fatty acid found in ruminant products such as milk and beef, has been shown to possess anti-cancer activities in in vivo animal models and in vitro cell culture systems. In human breast cancer, the overall duration of estrogen exposure is the most important risk factor for developing estrogen-responsive breast cancer. Accordingly, it has been suggested that estrogen exposure reduces apoptosis through the up-regulation of the anti-apoptosis protein, Bcl-2. Bcl-2, an anti-apoptotic protein, regulates apoptosis and plays a crucial role in the development and growth regulation of normal and cancerous cells. Our research interest is to examine the effects of CLA on the induction of apoptosis in human breast tissues.
methodsThe localization of Bcl-2 in both normal and cancerous human breast tissues was determined by immunohistochemical staining and the Bcl-2 protein expression was tested by western blot analysis. Co-culture of epithelial cells and stromal cells was carried out in the presence or absence of CLA to evaluate apoptosis in the context of a cell-cell interaction.
resultsThe results showed that both normal and cancerous breast tissues were positive for Bcl-2 staining, which was higher overall in mammary ducts but very low in the surrounding stromal compartment. Interestingly, by quantifying the western blot data, basal Bcl-2 protein levels were higher in normal breast epithelial cells than in cancerous epithelial cells. Furthermore, treatment with 17beta-estradiol (E2) stimulated growth and up-regulated Bcl-2 expression in estrogen responsive breast epithelial cells; however, these carcinogenic effects were diminished by either CLA or 4-Hydroxytamoxifen (Tam) and were suppressed further by the combination of CLA and Tam. In both one cell type cultured and co-culture systems, CLA induced cell apoptosis in ERalpha transfected MDA-MB-231 cells but not in the wild type MDA-MB-231 cells.
conclusionThese data, therefore, demonstrate that ERalpha plays important roles in CLA induced apoptosis in human breast tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.