ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008
Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line.G3 (Bethesda, Md.) · 2023Article
- Genome-wide simple sequence repeats (SSR) markers discovered from whole-genome sequence comparisons of multiple spinach accessions.Scientific reports · 2021Article
- Genome-wide analysis of microsatellite and sex-linked marker identification in Gleditsia sinensis.BMC plant biology · 2020Article
- Identification of QTLs associated with curd architecture in cauliflower.BMC plant biology · 2020Article
- The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers.PloS one · 2018Article
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- Developing genome-wide microsatellite markers of bamboo and their applications on molecular marker assisted taxonomy for accessions in the genus Phyllostachys.Scientific reports · 2015Article
- Recent progress in the use of 'omics technologies in brassicaceous vegetables.Frontiers in plant science · 2015Review
- Article
- Exploiting Illumina sequencing for the development of 95 novel polymorphic EST-SSR markers in common vetch (Vicia sativa subsp. sativa).Molecules (Basel, Switzerland) · 2014Article
- Transcriptome sequencing of two parental lines of cabbage (Brassica oleracea L. var. capitata L.) and construction of an EST-based genetic map.BMC genomics · 2014Article
- Genome-wide microsatellite characterization and marker development in the sequenced Brassica crop species.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Development and characterization of simple sequence repeat markers providing genome-wide coverage and high resolution in maize.DNA research : an international journal for rapid publication of reports on genes and genomes · 2013Article
- Development of a core set of single-locus SSR markers for allotetraploid rapeseed (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Genetic linkage maps for Asian and American lotus constructed using novel SSR markers derived from the genome of sequenced cultivar.BMC genomics · 2012Article
- Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata).BMC genomics · 2012Article
- Assigning Brassica microsatellite markers to the nine C-genome chromosomes using Brassica rapa var. trilocularis-B. oleracea var. alboglabra monosomic alien addition lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa-B. oleracea monosomic alien addition lines.Annals of botany · 2012Article
- Exploiting BAC-end sequences for the mining, characterization and utility of new short sequences repeat (SSR) markers in Citrus.Molecular biology reports · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The traditional development of simple sequence repeat (SSR) or microsatellite markers by probe hybridization can be time-consuming and requires the use of specialized laboratory equipment. In this study, probe hybridization was circumvented by using sequence information on 3,500 genomic clones mainly from Brassica oleracea to identify di, tri, tetra and penta-nucleotide repeats. A total of 587 primer pairs flanking SSR were developed using this approach. From these, 420 SSR markers amplified DNA in two parental lines of B. rapa (26% were polymorphic) and 523 in two parental lines of B. oleracea (32% were polymorphic). A diverse array of motif types was identified, characterized and compared with traditional SSR detection methods. The most abundant motifs found were di- (38%) and trinucleotides (33%) followed by penta- (16%) and tetranucleotide (13%) motifs. The type of motif class, motif length and repeat were not indicative of polymorphisms. The frequency of B. oleracea SSRs in genomic shotgun sequence was estimated to be 1 every 4 Kb. In general, the average motif length and repeat numbers were shorter than those obtained previously by probe hybridization, and they contained a more balanced representation of SSR motif types in the genome by identifying those that do not hybridize well to DNA probes. Brassica genomic DNA sequence information is a promising resource for developing a large number of SSR molecular markers in Brassica species.
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