ArticleJournal of dental research2008
Clarithromycin transport by gingival fibroblasts and epithelial cells.
Article in Journal of dental research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- [Efficacy of clarithromycin in the adjuvant treatment of chronic periodontitis: a Meta-analysis].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2020Pooled it
- Effect of gingivitis on azithromycin concentrations in gingival crevicular fluid.Journal of periodontology · 2012Trial
- Distribution of systemic clarithromycin to gingiva.Journal of periodontology · 2008Trial
- Effectiveness of antibacterial agents against cell-invading bacteria such as Streptococcus pyogenes and Haemophilus influenzae.BMC microbiology · 2021Article
- Clinical study on natural gingival color.Odontology · 2019Article
- Azithromycin suppresses P. gingivalis LPS-induced pro-inflammatory cytokine and chemokine production by human gingival fibroblasts in vitro.Clinical oral investigations · 2015Article
- Azithromycin kills invasive Aggregatibacter actinomycetemcomitans in gingival epithelial cells.Antimicrobial agents and chemotherapy · 2013Article
- Azithromycin concentrations in blood and gingival crevicular fluid after systemic administration.Journal of periodontology · 2011Article
- Clarithromycin accumulation by phagocytes and its effect on killing of Aggregatibacter actinomycetemcomitans.Journal of periodontology · 2011Article
- Azithromycin decreases crevicular fluid volume and mediator content.Journal of dental research · 2010Article
- Evaluating the effectiveness of clarithromycin as an adjunct to scaling and root planing: A randomized clinical trial.Journal of Indian Society of PeriodontologyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Macrolide antibiotics penetrate cells, but the mechanism by which this occurs is unclear. The objective of this study was to characterize the mechanisms of clarithromycin uptake by gingival fibroblasts and oral epithelium. Cultured human gingival fibroblasts and SCC-25 cells were incubated with [(3)H]-clarithromycin. We assayed clarithromycin transport by measuring cell-associated radioactivity over time. Fibroblasts and epithelial cells rapidly accumulated clarithromycin, attaining steady-state intracellular concentrations within 15 minutes. Incubation in medium containing 2 mug/mL clarithromycin yielded steady-state intracellular concentrations of 75.8 mug/mL in fibroblasts and 6.6 mug/mL in SCC-25 cells. Clarithromycin transport exhibited Michaelis-Menten kinetics and was inhibited below 37 degrees C. The Michaelis constants for fibro-blasts and SCC-25 cells were 78.4 and 227 mug/mL, respectively, while the maximum transport velocities were 264 and 381 ng/min/10(6) cells, respectively. Thus, both types of cells take up clarithromycin via a concentrative active transport system. By increasing intracellular clarithromycin levels, this system may enhance the effectiveness of clarithromycin against invasive periodontal pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.