ArticleJournal of clinical microbiology1991
Construction of a DNA probe and detection of Actinobacillus pleuropneumoniae by using polymerase chain reaction.
Article in Journal of clinical microbiology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 38 citations in OpenAlex.
- Novel DNA Markers for Identification of Actinobacillus pleuropneumoniae.Microbiology spectrum · 2022Article
- Evaluation and field validation of PCR tests for detection of Actinobacillus pleuropneumoniae in subclinically infected pigs.Journal of clinical microbiology · 2003Article
- Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6.Journal of clinical microbiology · 2003Article
- Pasteurellaceae isolated from tonsillar samples of commercially-reared American bison (Bison bison).Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
- Genomic relatedness among Actinobacillus pleuropneumoniae field strains of sterotypes 1 and 5 isolated from healthy and diseased pigs.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
- Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification.Journal of clinical microbiology · 1999Article
- Detection and identification of Actinobacillus pleuropneumoniae serotype 5 by multiplex PCR.Journal of clinical microbiology · 1998Article
- Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein.Journal of clinical microbiology · 1998Article
- Applications of DNA amplification techniques in veterinary diagnostics.Veterinary research communications · 1995Review
- Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis.Journal of clinical microbiology · 1994Article
- Serotype identification of Actinobacillus pleuropneumoniae by arbitrarily primed polymerase chain reaction.Journal of clinical microbiology · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 1.5-kb Actinobacillus pleuropneumoniae 4074 DNA fragment from a genomic library was found to hybridization. No cross-hybridization hybridization. No cross-hybridization was detected with DNAs from hemolytic members of the family Pasteurellaceae. From the nucleotide sequence of the putative genomic probe, three primers were synthesized for use in polymerase chain reactions (PCRs), with 31 strains tested by using purified and crude DNA targets. PCR amplification products of 610 and 985 bp were observed in nucleic acids extracted from the 12 known serotypes and a biotype 2 strain. Template DNAs from other gram-negative and gram-positive bacteria, some of them found in the normal flora of swine and the upper respiratory tract, were not amplified by PCR. The only exception was an amplification of a similar 610- or 985-bp sequence in Actinobacillus lignieresii, a species that is closely related to A. pleuropneumoniae but that has never been isolated from swine. Amplification of specific A. pleuropneumoniae sequences by PCR directly from clinical specimens may find applications in the identification of asymptomatic carriers as well as in efforts to eradicate porcine pleuropneumonia.
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Registered trials
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