ArticleFish physiology and biochemistry2008
Identification of Wap65, a human homologue of hemopexin as a copper-inducible gene in swordtail fish, Xiphophorus helleri.
Article in Fish physiology and biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 34 citations in OpenAlex.
- Characterization of carp seminal plasma Wap65-2 and its participation in the testicular immune response and temperature acclimation.Veterinary research · 2020Article
- Skin mucus proteins of lumpsucker (Biochemistry and biophysics reports · 2017Article
- The ribosomal protein L19 mRNA is induced by copper exposure in the swordtail fish, Xiphophorus helleri.Molecular biology reports · 2012Article
- Molecular cloning of liver Wap65 cDNA in ayu (Plecoglossus altivelis) and mRNA expression changes following Listonella anguillarum infection.Molecular biology reports · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
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Abstract
Copper is one of the major heavy metal pollutants found in the aquatic environment. Therefore, it is important for determining the genes that play a key role in copper metabolism in aquatic organisms. This study, thus, aimed to identify a new copper-inducible gene in swordtail fish, Xiphophorus helleri. Using ACP-based RT-PCR coupled with RLM-RACE, we cloned Wap65, a mammalian homologue of hemopexin gene. The gene exhibits high identity at amino acid levels with the Wap65 gene of other fish species (42-68%) and mammalian hemopexin gene (35-37%). In addition, ten cysteine and two histidine residues are conserved in the swordtail fish Wap65 gene. These cysteine residues are vital for structural integrity, and histidine residues provide high binding affinity towards heme. As revealed by RT-PCR, the gene was upregulated in swordtail fish that were exposed to copper in a dose- and time-dependent manner. Therefore, the identification of Wap65, a mammalian homologue of hemopexin, as a new copper-inducible gene will provide greater insight into the role of this gene in copper metabolism.
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