ArticleVirus genes2008
Identification and characterization of a novel thymidylate synthase from deep-sea thermophilic bacteriophage Geobacillus virus E2.
Article in Virus genes, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Article
- Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage.Scientific reports · 2016Article
- Genome sequence of a novel deep-sea vent epsilonproteobacterial phage provides new insight into the co-evolution of Epsilonproteobacteria and their phages.Extremophiles : life under extreme conditions · 2013Article
- Spatial distribution of viruses associated with planktonic and attached microbial communities in hydrothermal environments.Applied and environmental microbiology · 2012Article
- Genome analysis of deep-sea thermophilic phage D6E.Applied and environmental microbiology · 2010Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thymidylate synthase (TS) is essential for de novo synthesis of dTMP and is a key enzyme involved in DNA synthesis and transcriptional regulation of organisms. Due to their biologic importance, TSs have been intensively studied. In this investigation, a thermostable TS was identified from a deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2). It was demonstrated that GVE2-TS was highly homologous to known TSs and contained five characteristic conserved domains. The temporal analyses by Northern and Western blots revealed that the GVE2-TS was transcribed and expressed early after Geobacillus virus E2 infection, identifying it as a viral early gene. As shown by gel mobility shift assays, the recombinant GVE2-TS protein had the capacity to bind its own mRNA. Our study presented the first report on thymidylate synthase from deep-sea thermophilic bacteriophage.
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