ArticlePloS one2008
Specific and sensitive detection of H. pylori in biological specimens by real-time RT-PCR and in situ hybridization.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular characterization of Helicobacter pylori isolated from Nile Tilapia (Oreochromis niloticus) and fish handlers.BMC veterinary research · 2023Article
- The TNF-Alpha Inducing Protein is Associated With Gastric Inflammation and Hyperplasia in a Murine Model ofFrontiers in pharmacology · 2022Article
- MixedHealthcare (Basel, Switzerland) · 2019Article
- Both diet and Helicobacter pylori infection contribute to atherosclerosis in pre- and postmenopausal cynomolgus monkeys.PloS one · 2019Article
- Article
- The Gastric and Intestinal Microbiome: Role of Proton Pump Inhibitors.Current gastroenterology reports · 2017Review
- Comparative Evaluation of RUT, PCR and ELISA Tests for Detection of Infection with Cytotoxigenic H. pylori.Advanced pharmaceutical bulletin · 2016Article
- Dyspepsia: When and How to Test for Helicobacter pylori Infection.Gastroenterology research and practice · 2016Review
- Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth.Mucosal immunology · 2015Article
- Helicobacter pylori and oral pathology: relationship with the gastric infection.World journal of gastroenterology · 2014Article
- Mutations to essential orphan response regulator HP1043 of Helicobacter pylori result in growth-stage regulatory defects.Infection and immunity · 2013Article
- Article
- State of the globe: Diagnostic tests to detect Helicobacter pylori tonsillitis.Journal of global infectious diseases · 2012Article
- Agreement rate of rapid urease test, conventional PCR, and scorpion real-time PCR in detecting helicobacter pylori from tonsillar samples of patients with chronic tonsillitis.Journal of global infectious diseases · 2012Article
- Mechanism of H. pylori intracellular entry: an in vitro study.Frontiers in cellular and infection microbiology · 2012Article
- Article
- Culture Method and PCR for the Detection of Helicobacter pylori in Drinking Water in Basrah Governorate Iraq.Gastroenterology research and practice · 2012Article
- Simultaneous amplification of two bacterial genes: more reliable method of Helicobacter pylori detection in microbial rich dental plaque samples.Current microbiology · 2011Article
- Relationship between H.Pylori infection and clinicopathological features and prognosis of gastric cancer.BMC cancer · 2010Article
- Treatment outcome of localized Helicobacter pylori-negative low-grade gastric MALT lymphoma.World journal of gastroenterology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
PCR detection of H. pylori in biological specimens is rendered difficult by the extensive polymorphism of H. pylori genes and the suppressed expression of some genes in many strains. The goal of the present study was to (1) define a domain of the 16S rRNA sequence that is both highly conserved among H. pylori strains and also specific to the species, and (2) to develop and validate specific and sensitive molecular methods for the detection of H. pylori. We used a combination of in silico and molecular approaches to achieve sensitive and specific detection of H. pylori in biologic media. We sequenced two isolates from patients living in different continents and demonstrated that a 546-bp domain of the H. pylori 16S rRNA sequence was conserved in those strains and in published sequences. Within this conserved sequence, we defined a 229-bp domain that is 100% homologous in most H. pylori strains available in GenBank and also is specific for H. pylori. This sub-domain was then used to design (1) a set of high quality RT-PCR primers and probe that encompassed a 76-bp sequence and included at least two mismatches with other Helicobacter sp. 16S rRNA; and (2) in situ hybridization antisense probes. The sensitivity and specificity of the approaches were then demonstrated by using gastric biopsy specimens from patients and rhesus monkeys. This H. pylori-specific region of the 16S rRNA sequence is highly conserved among most H. pylori strains and allows specific detection, identification, and quantification of this bacterium in biological specimens.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.