ArticleRNA (New York, N.Y.)2008
A new regulatory circuit in ribosomal protein operons: S2-mediated control of the rpsB-tsf expression in vivo.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 62 citations in OpenAlex.
- Revealing global stoichiometry conservation architecture in cells from Raman spectral patterns.eLife · 2026Article
- Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023.International journal of molecular sciences · 2024Review
- Structural insight into translation initiation of thebioRxiv : the preprint server for biology · 2023Article
- Quiescence ofMicroorganisms · 2023Article
- Amyloidogenic Peptides: New Class of Antimicrobial Peptides with the Novel Mechanism of Activity.International journal of molecular sciences · 2022Review
- Autogenous regulation in vivo of theRNA (New York, N.Y.) · 2020Article
- RNA base-pairing complexity in living cells visualized by correlated chemical probing.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A group II intron-encoded protein interacts with the cellular replicative machinery through the β-sliding clamp.Nucleic acids research · 2019Article
- Deciphering global gene expression and regulation strategy in Escherichia coli during carbon limitation.Microbial biotechnology · 2019Article
- Article
- Bi-allelic Mutations in the Mitochondrial Ribosomal Protein MRPS2 Cause Sensorineural Hearing Loss, Hypoglycemia, and Multiple OXPHOS Complex Deficiencies.American journal of human genetics · 2018Article
- rRNA Mimicry in RNA Regulation of Gene Expression.Microbiology spectrum · 2018Review
- Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei.Scientific reports · 2017Article
- Metal nanoparticles: understanding the mechanisms behind antibacterial activity.Journal of nanobiotechnology · 2017Review
- Regulation of Ribosomal Protein Operons rplM-rpsI, rpmB-rpmG, and rplU-rpmA at the Transcriptional and Translational Levels.Journal of bacteriology · 2016Article
- Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.Journal of bacteriology · 2016Article
- An S6:S18 complex inhibits translation of E. coli rpsF.RNA (New York, N.Y.) · 2015Article
- Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria.RNA (New York, N.Y.) · 2015Article
- Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate.BMC microbiology · 2014Article
- Validation of a fluorescence-based screening concept to identify ribosome assembly defects in Escherichia coli.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autogenous regulation is a general strategy of balancing ribosomal protein synthesis in bacteria. Control mechanisms have been studied in detail for most of ribosomal protein operons, except for rpsB-tsf encoding essential r-protein S2 and elongation factor Ts, where even the promoter has remained unknown. By using single-copy translational fusions with the chromosomal lacZ gene and Western-blot analysis, we demonstrate here that S2 serves as a negative regulator of both rpsB and tsf expression in vivo, acting at a single target within the rpsB 5'-untranslated region (5'-UTR). As determined by primer extension, transcription of the Escherichia coli rpsB-tsf operon starts 162 nucleotides upstream of the rpsB initiation codon at a single promoter TGTGGTATAAA belonging to the extended -10 promoter class. Both the promoter signature and the 5'-UTR structure of the rpsB gene appear to be highly conserved in gamma-proteobacteria. Deletion analysis of the rpsB 5'-UTR within rpsB'-'lacZ fusions has revealed that an operator region involved in the S2 autoregulation comprises conserved structural elements located upstream of the rpsB ribosome binding site. The S2-mediated autogenous control is impaired in rpsB mutants and, more surprisingly, in the rpsA mutant producing decreased amounts of truncated r-protein S1 (rpsAIS10), indicating that S2 might act as a repressor in cooperation with S1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.