ArticleProceedings of the National Academy of Sciences of the United States of America2008
Evolution of sequence specificity in a restriction endonuclease by a point mutation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- DNA Flap-Mediated Control of Transcription for Programmable RNA Synthesis.Angewandte Chemie (International ed. in English) · 2026Article
- Modular determinants of cleavage preference in GIY-YIG nucleases revealed by block-based DNA shuffling and directed evolution.Nucleic acids research · 2026Article
- The defensome of complex bacterial communities.Nature communications · 2024Article
- Modified nucleoside triphosphates in bacterial research forRSC chemical biology · 2020Article
- Promiscuous DNA cleavage by HpyAII endonuclease is modulated by the HNH catalytic residues.Bioscience reports · 2020Article
- Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases.Nucleic acids research · 2018Article
- The ascent of man(made oxidoreductases).Current opinion in structural biology · 2018Review
- Restriction endonuclease triggered bacterial apoptosis as a mechanism for long time survival.Nucleic acids research · 2017Article
- Increasing cleavage specificity and activity of restriction endonuclease KpnI.Nucleic acids research · 2013Article
- Massively parallel characterization of restriction endonucleases.Nucleic acids research · 2013Article
- Diverse functions of restriction-modification systems in addition to cellular defense.Microbiology and molecular biology reviews : MMBR · 2013Review
- Promiscuous restriction is a cellular defense strategy that confers fitness advantage to bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Directed enzyme evolution: climbing fitness peaks one amino acid at a time.Current opinion in chemical biology · 2009Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restriction endonucleases (REases) protect bacteria from invading foreign DNAs and are endowed with exquisite sequence specificity. REases have originated from the ancestral proteins and evolved new sequence specificities by genetic recombination, gene duplication, replication slippage, and transpositional events. They are also speculated to have evolved from nonspecific endonucleases, attaining a high degree of sequence specificity through point mutations. We describe here an example of generation of exquisitely site-specific REase from a highly-promiscuous one by a single point mutation.
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