ArticleMolecular microbiology2008
Erythromycin-induced ribosome stalling and RNase J1-mediated mRNA processing in Bacillus subtilis.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Metastable Folding of Bacillus subtilis glmS Ribozyme Modulates Turnover by RNase J1.Journal of molecular biology · 2026Article
- 16-membered ring macrolides and erythromycin induce ermB expression by different mechanisms.BMC microbiology · 2022Article
- Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome.Nature communications · 2019Article
- Mechanistic Insights into Cofactor-Dependent Coupling of RNA Folding and mRNA Transcription/Translation by a Cobalamin Riboswitch.Cell reports · 2016Article
- Complex Regulation Pathways of AmpC-Mediated β-Lactam Resistance in Enterobacter cloacae Complex.Antimicrobial agents and chemotherapy · 2015Article
- Initiation of mRNA decay in bacteria.Cellular and molecular life sciences : CMLS · 2014Review
- Acquired inducible antimicrobial resistance in Gram-positive bacteria.Future microbiology · 2012Review
- The ribosomal exit tunnel as a target for optimizing protein expression in Escherichia coli.Biotechnology journal · 2012Article
- Analysis of aminoacyl- and peptidyl-tRNAs by gel electrophoresis.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Decay of a model mRNA in Bacillus subtilis by a combination of RNase J1 5' exonuclease and RNase Y endonuclease activities.Journal of bacteriology · 2011Article
- An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.Molecular cell · 2011Article
- Enterococcus faecalis rnjB is required for pilin gene expression and biofilm formation.Journal of bacteriology · 2010Article
- An overview of RNAs with regulatory functions in gram-positive bacteria.Cellular and molecular life sciences : CMLS · 2010Review
- Bacillus subtilis RNase J1 endonuclease and 5' exonuclease activities in the turnover of DeltaermC mRNA.RNA (New York, N.Y.) · 2009Article
- RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilis.The EMBO journal · 2009Article
- Processing and stability of inducibly expressed rpsO mRNA derivatives in Bacillus subtilis.Journal of bacteriology · 2009Article
- RNase II is important for A-site mRNA cleavage during ribosome pausing.Molecular microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
summaryAddition of erythromycin (Em) to a Bacillus subtilis strain carrying the ermC gene results in ribosome stalling in the ermC leader peptide coding sequence. Using DeltaermC, a deletion derivative of ermC that specifies the 254 nucleotide DeltaermC mRNA, we showed previously that ribosome stalling is concomitant with processing of DeltaermC mRNA, generating a 209 nucleotide RNA whose 5' end maps to codon 5 of the DeltaermC coding sequence. Here we probed for peptidyl-tRNA to show that ribosome stalling occurs after incorporation of the amino acid specified by codon 9. Thus, cleavage upstream of codon 5 is not an example of 'A-site cleavage' that has been reported for Escherichia coli. Analysis of DeltaermC mRNA processing in endoribonuclease mutant strains showed that this processing is RNase J1-dependent. DeltaermC mRNA processing was inhibited by the presence of stable secondary structure at the 5' end, demonstrating 5'-end dependence, and was shown to be a result of RNase J1 endonuclease activity, rather than 5'-to-3' exonuclease activity. Examination of processing in derivatives of DeltaermC that had codons inserted upstream of the ribosome stalling site revealed that Em-induced ribosome stalling can occur considerably further from the start codon than would be expected based on previous studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.