ArticleMolecular and cellular biology2008
Cotranscriptional splicing potentiates the mRNA production from a subset of estradiol-stimulated genes.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Escorting mRNAs - the multifunctional and divergent roles of cap-chaperones in post-transcriptional gene expression.Journal of cell science · 2026Review
- Distinct biogenesis pathways may have led to functional divergence of the human and Drosophila Arglu1 sisRNA.EMBO reports · 2023Article
- The nuclear cap-binding complex as choreographer of gene transcription and pre-mRNA processing.Genes & development · 2020Review
- An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.Cell research · 2018Article
- Article
- RNA splicing regulates the temporal order of TNF-induced gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Interplay between estrogen receptor and AKT in estradiol-induced alternative splicing.BMC medical genomics · 2013Article
- The RNA helicase DDX5/p68 is a key factor promoting c-fos expression at different levels from transcription to mRNA export.Nucleic acids research · 2013Article
- G9a functions as a molecular scaffold for assembly of transcriptional coactivators on a subset of glucocorticoid receptor target genes.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Splicing switch of an epigenetic regulator by RNA helicases promotes tumor-cell invasiveness.Nature structural & molecular biology · 2012Article
- Dual role of the ddx5/ddx17 RNA helicases in the control of the pro-migratory NFAT5 transcription factor.Oncogene · 2012Article
- Cotranscriptional exon skipping in the genotoxic stress response.Nature structural & molecular biology · 2010Article
- Nuclear networking fashions pre-messenger RNA and primary microRNA transcripts for function.Trends in cell biology · 2010Review
- Subnuclear compartmentalization of transiently expressed polyadenylated pri-microRNAs: processing at transcription sites or accumulation in SC35 foci.Cell cycle (Georgetown, Tex.) · 2009Article
- Where splicing joins chromatin.Nucleus (Austin, Tex.)Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While early steps of gene expression, such as transcription preinitiation, are known to often be rate limiting and to be regulated by such stimuli as steroid hormones, the potential impact of downstream steps, including splicing, on the mRNA production rate is unknown. In this work, we studied the effects of the transcriptional stimulus estradiol on cyclin D1, PS2, and c-fos gene expression by measuring the levels of RNA polymerase II on the DNA templates, the levels of nascent transcripts associated with RNA polymerase II, and the levels of unspliced, partially spliced, and fully spliced RNAs. We demonstrated that the efficiency of cotranscriptional splicing of the first intron was higher in the case of cyclin D1 than with PS2 and potentiated the cyclin D1 mRNA production rate. The mechanism involved in cotranscriptional splicing depended on the level of serine 5 phosphorylation of RNA polymerase II at the gene 5' end and on the recruitment of CBP80, one of the two subunits of the cap binding complex, which stimulates splicing of the promoter-proximal intron. Our data indicate that mRNA production from a subset of estradiol-stimulated genes, such as cyclin D1, could occur in a very efficient "assembly line." In contrast, we demonstrated for the first time that despite a strong transcriptional activation of the PS2 gene, the production of mRNA is not optimized owing to inefficient cotranscriptional RNA processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.