ArticleJournal of molecular biology2008
Visualization of the eEF2-80S ribosome transition-state complex by cryo-electron microscopy.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.Protein science : a publication of the Protein Society · 2026Article
- Elongation factor 2 in cancer: a promising therapeutic target in protein translation.Cellular & molecular biology letters · 2024Review
- Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.Nature communications · 2021Article
- Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation.The EMBO journal · 2021Article
- Joachim Frank's Binding with the Ribosome.Structure (London, England : 1993) · 2019Review
- Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.eLife · 2016Article
- Conjugation with polyamines enhances the antibacterial and anticancer activity of chloramphenicol.Nucleic acids research · 2014Article
- Structure of EF-G-ribosome complex in a pretranslocation state.Nature structural & molecular biology · 2013Article
- Functional replacement of two highly conserved tetraloops in the bacterial ribosome.Biochemistry · 2012Article
- The structure and function of the eukaryotic ribosome.Cold Spring Harbor perspectives in biology · 2012Review
- Consensus among flexible fitting approaches improves the interpretation of cryo-EM data.Journal of structural biology · 2012Article
- Almost lost in translation. Cryo-EM of a dynamic macromolecular complex: the ribosome.European biophysics journal : EBJ · 2011Review
- The mechanism for activation of GTP hydrolysis on the ribosome.Science (New York, N.Y.) · 2010Article
- From DNA to proteins via the ribosome: structural insights into the workings of the translation machinery.Human genomics · 2010Review
- Single-particle reconstruction of biological macromolecules in electron microscopy--30 years.Quarterly reviews of biophysics · 2009Review
- Conformational changes in switch I of EF-G drive its directional cycling on and off the ribosome.The EMBO journal · 2009Article
- Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
In an attempt to understand ribosome-induced GTP hydrolysis on eEF2, we determined a 12.6-A cryo-electron microscopy reconstruction of the eEF2-bound 80S ribosome in the presence of aluminum tetrafluoride and GDP, with aluminum tetrafluoride mimicking the gamma-phosphate during hydrolysis. This is the first visualization of a structure representing a transition-state complex on the ribosome. Tight interactions are observed between the factor's G domain and the large ribosomal subunit, as well as between domain IV and an intersubunit bridge. In contrast, some of the domains of eEF2 implicated in small subunit binding display a large degree of flexibility. Furthermore, we find support for a transition-state model conformation of the switch I region in this complex where the reoriented switch I region interacts with a conserved rRNA region of the 40S subunit formed by loops of the 18S RNA helices 8 and 14. This complex is structurally distinct from the eEF2-bound 80S ribosome complexes previously reported, and analysis of this map sheds light on the GTPase-coupled translocation mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.