ArticleNature genetics2008
Dynamic transcriptome of Schizosaccharomyces pombe shown by RNA-DNA hybrid mapping.
Article in Nature genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
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Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Podbat: a novel genomic tool reveals Swr1-independent H2A.Z incorporation at gene coding sequences through epigenetic meta-analysis.PLoS computational biology · 2011Pooled it
- R-loop landscapes in the developing human brain are linked to neural differentiation and cell type-specific transcription.Translational psychiatry · 2026Article
- Histone deacetylation as a landmark for Sgo2 relocation from centromeres to subtelomeres during interphase.iScience · 2025Article
- S9.6-based hybrid capture immunoassay for pathogen detection.Scientific reports · 2023Article
- S9.6 Antibody-Enzyme Conjugates for the Detection of DNA-RNA Hybrids.Bioconjugate chemistry · 2023Article
- Structural basis of R-loop recognition by the S9.6 monoclonal antibody.Nature communications · 2022Article
- lncRNA transcription induces meiotic recombination through chromatin remodelling in fission yeast.Communications biology · 2021Article
- Genome-wide identification and functional characterization of natural antisense transcripts in Salvia miltiorrhiza.Scientific reports · 2021Article
- An RNA aptamer to HP1/Swi6 facilitates heterochromatin formation at an ectopic locus in S.pombe.RNA biology · 2019Article
- Comprehensive profiling of the fission yeast transcription start site activity during stress and media response.Nucleic acids research · 2019Article
- A current view on long noncoding RNAs in yeast and filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Long noncoding RNA repertoire and targeting by nuclear exosome, cytoplasmic exonuclease, and RNAi in fission yeast.RNA (New York, N.Y.) · 2018Article
- Native elongating transcript sequencing reveals global anti-correlation between sense and antisense nascent transcription in fission yeast.RNA (New York, N.Y.) · 2018Article
- The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences.PloS one · 2017Article
- Determinants of RNA metabolism in the Schizosaccharomyces pombe genome.Molecular systems biology · 2016Article
- The Antisense Transcriptome and the Human Brain.Journal of molecular neuroscience : MN · 2016Review
- Proteomic Analysis of Dhh1 Complexes Reveals a Role for Hsp40 Chaperone Ydj1 in Yeast P-Body Assembly.G3 (Bethesda, Md.) · 2015Article
- An antisense RNA-mediated mechanism eliminates a meiosis-specific copper-regulated transcript in mitotic cells.The Journal of biological chemistry · 2015Article
- Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast.Nature communications · 2015Article
- Fission yeast Cdk7 controls gene expression through both its CAK and C-terminal domain kinase activities.Molecular and cellular biology · 2015Article
5 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
We have determined the high-resolution strand-specific transcriptome of the fission yeast S. pombe under multiple growth conditions using a novel RNA-DNA hybridization mapping (HybMap) technique. HybMap uses an antibody against an RNA-DNA hybrid to detect RNA molecules hybridized to a high-density DNA oligonucleotide tiling microarray. HybMap showed exceptional dynamic range and reproducibility, and allowed us to identify strand-specific coding, noncoding and structural RNAs, as well as previously unknown RNAs conserved in distant yeast species. Notably, we found that virtually the entire euchromatic genome (including intergenics) is transcribed, with heterochromatin dampening intergenic transcription. We identified features including large numbers of condition-specific noncoding RNAs, extensive antisense transcription, new properties of antisense transcripts and induced divergent transcription. Furthermore, our HybMap data informed the efficiency and locations of RNA splicing genome-wide. Finally, we observed strand-specific transcription islands around tRNAs at heterochromatin boundaries inside centromeres. Here, we discuss these new features in terms of organism fitness and transcriptome evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.