ArticleApplied and environmental microbiology2008
Cloning and overexpression of alkaline phosphatase PhoK from Sphingomonas sp. strain BSAR-1 for bioprecipitation of uranium from alkaline solutions.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Conserved regions and molecular cloning of Acid and Alkaline phosphatases in Streptomyces sp. MMA-NRC.Scientific reports · 2026Article
- Broad Substrate Specificity and High Catalytic Activity ofACS omega · 2025Article
- Uranium contamination mediating soil and ore microbial community assembly at four mining sites, South China.Frontiers in microbiology · 2025Article
- Recycled Household Ash in Rice Paddies of Bangladesh for Sustainable Production of Rice Without Altering Grain Arsenic and Cadmium.Exposure and health · 2024Article
- Impact of microbial processes on the safety of deep geological repositories for radioactive waste.Frontiers in microbiology · 2023Review
- Isolation and characterization of a recombinant class C acid phosphatase fromBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Molecular Mechanisms Underlying Bacterial Uranium Resistance.Frontiers in microbiology · 2022Review
- uPIC-M: Efficient and Scalable Preparation of Clonal Single Mutant Libraries for High-Throughput Protein Biochemistry.ACS omega · 2021Article
- Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications.Microbial biotechnology · 2021Review
- Emerging investigator series: entrapment of uranium-phosphorus nanocrystals inside root cells ofEnvironmental science. Processes & impacts · 2021Article
- Article
- Effects of PVP-coated silver nanoparticles on enzyme activity, bacterial and archaeal community structure and function in a yellow-brown loam soil.Environmental science and pollution research international · 2020Article
- Is Long-Term Heavy Metal Exposure Driving Carriage of Antibiotic Resistance in Environmental Opportunistic Pathogens: A Comprehensive Phenomic and Genomic Assessment UsingFrontiers in microbiology · 2020Article
- Characterization of arsenic oxidation and uranium bioremediation potential of arsenic resistant bacteria isolated from uranium ore.Environmental science and pollution research international · 2019Article
- Evaluation of Natural and Synthetic Phosphate Donors for the Improved Enzymatic Synthesis of Phosphate Monoesters.Advanced synthesis & catalysis · 2018Article
- Unusual Versatility of the Filamentous, Diazotrophic Cyanobacterium Anabaena torulosa Revealed for Its Survival during Prolonged Uranium Exposure.Applied and environmental microbiology · 2017Article
- Exploring the Impacts of Anthropogenic Disturbance on Seawater and Sediment Microbial Communities in Korean Coastal Waters Using Metagenomics Analysis.International journal of environmental research and public health · 2017Article
- Article
- Interaction of Uranium with Bacterial Cell Surfaces: Inferences from Phosphatase-Mediated Uranium Precipitation.Applied and environmental microbiology · 2016Article
- In silico analysis and experimental validation of lipoprotein and novel Tat signal peptides processing in Anabaena sp. PCC7120.Journal of microbiology (Seoul, Korea) · 2015Article
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4 authors.
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Abstract
Cells of Sphingomonas sp. strain BSAR-1 constitutively expressed an alkaline phosphatase, which was also secreted in the extracellular medium. A null mutant lacking this alkaline phosphatase activity was isolated by Tn5 random mutagenesis. The corresponding gene, designated phoK, was cloned and overexpressed in Escherichia coli strain BL21(DE3). The resultant E. coli strain EK4 overexpressed cellular activity 55 times higher and secreted extracellular PhoK activity 13 times higher than did BSAR-1. The recombinant strain very rapidly precipitated >90% of input uranium in less than 2 h from alkaline solutions (pH, 9 +/- 0.2) containing 0.5 to 5 mM of uranyl carbonate, compared to BSAR-1, which precipitated uranium in >7 h. In both strains BSAR-1 and EK4, precipitated uranium remained cell bound. The EK4 cells exhibited a much higher loading capacity of 3.8 g U/g dry weight in <2 h compared to only 1.5 g U/g dry weight in >7 h in BSAR-1. The data demonstrate the potential utility of genetically engineering PhoK for the bioprecipitation of uranium from alkaline solutions.
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