ArticleNucleic acids research2008
Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 135 citations in OpenAlex.
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- Zinc Finger Artificial Transcription Factor-Mediated Chloroplast Genome Interrogation in Arabidopsis thaliana.Plant & cell physiology · 2019Article
- Programmable T7-based synthetic transcription factors.Nucleic acids research · 2018Article
- Exploring of the feature space of de novo developed post-transcriptional riboregulators.PLoS computational biology · 2018Article
- Construction of enhanced transcriptional activators for improving cellulase production inBioresources and bioprocessing · 2018Article
- Enhancement of Arabidopsis growth characteristics using genome interrogation with artificial transcription factors.PloS one · 2017Article
- Directed Evolution of Dunaliella salina Ds-26-16 and Salt-Tolerant Response in Escherichia coli.International journal of molecular sciences · 2016Article
- Using global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis.Microbial cell factories · 2016Article
- Rational selection and engineering of exogenous principal sigma factor (σ(HrdB)) to increase teicoplanin production in an industrial strain of Actinoplanes teichomyceticus.Microbial cell factories · 2014Article
- Improved production of L-threonine in Escherichia coli by use of a DNA scaffold system.Applied and environmental microbiology · 2013Article
- Combinatorial approaches for inverse metabolic engineering applications.Computational and structural biotechnology journal · 2012Review
- Enhancing E. coli tolerance towards oxidative stress via engineering its global regulator cAMP receptor protein (CRP).PloS one · 2012Article
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- Strain engineering for improved expression of recombinant proteins in bacteria.Microbial cell factories · 2011Review
- A multi-functional synthetic gene network: a frequency multiplier, oscillator and switch.PloS one · 2011Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Now that many genomes have been sequenced and the products of newly identified genes have been annotated, the next goal is to engineer the desired phenotypes in organisms of interest. For the phenotypic engineering of microorganisms, we have developed novel artificial transcription factors (ATFs) capable of reprogramming innate gene expression circuits in Escherichia coli. These ATFs are composed of zinc finger (ZF) DNA-binding proteins, with distinct specificities, fused to an E. coli cyclic AMP receptor protein (CRP). By randomly assembling 40 different types of ZFs, we have constructed more than 6.4 x 10(4) ATFs that consist of 3 ZF DNA-binding domains and a CRP effector domain. Using these ATFs, we induced various phenotypic changes in E. coli and selected for industrially important traits, such as resistance to heat shock, osmotic pressure and cold shock. Genes associated with the heat-shock resistance phenotype were then characterized. These results and the general applicability of this platform clearly indicate that novel ATFs are powerful tools for the phenotypic engineering of microorganisms and can facilitate microbial functional genomic studies.
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Registered trials
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