ArticleInternational journal of bioinformatics research and applications2008
Computationally Optimised DNA Assembly of synthetic genes.
Article in International journal of bioinformatics research and applications, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis.Microbial cell factories · 2024Review
- Article
- In vitro targeting of strand transfer by the Ty3 retroelement integrase.The Journal of biological chemistry · 2012Article
- Incorporation of a horizontally transferred gene into an operon during cnidarian evolution.PloS one · 2012Article
- A vector set for systematic metabolic engineering in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2011Article
- All-codon scanning identifies p53 cancer rescue mutations.Nucleic acids research · 2010Article
- High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides.Nucleic acids research · 2010Article
- Mechanism of U insertion RNA editing in trypanosome mitochondria: the bimodal TUTase activity of the core complex.Journal of molecular biology · 2010Article
- Making green.Nature biotechnology · 2009Article
- Novel TUTase associates with an editosome-like complex in mitochondria of Trypanosoma brucei.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Gene synthesis is hampered by two obstacles: improper assembly of oligonucleotides; oligonucleotide defects incurred during chemical synthesis. To overcome the first problem, we describe the employment of a Computationally Optimised DNA Assembly (CODA) algorithm that uses the degeneracy of the genetic code to design overlapping oligonucleotides with thermodynamic properties for self-assembly into a single, linear, DNA product. To address the second problem, we describe a hierarchical assembly strategy that reduces the incorporation of defective oligonucleotides into full-length gene constructs. The CODA algorithm and these biological methods enable fast, simple and reliable assemblies of sequence-correct full-length genes.
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Registered trials
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