ArticleComparative biochemistry and physiology. Part A, Molecular & integrative physiology2008
Effects of imaging conditions on mitochondrial transport and length in larval motor axons of Drosophila.
Article in Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Drosophila motor neuron boutons remodel through membrane blebbing coupled with muscle contraction.Nature communications · 2023Article
- Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The role of mitochondria in axon development and regeneration.Developmental neurobiology · 2018Review
- Compartmentalized Regulation of Parkin-Mediated Mitochondrial Quality Control in the Drosophila Nervous System In Vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Inner membrane fusion mediates spatial distribution of axonal mitochondria.Scientific reports · 2016Article
- The Organization of Mitochondrial Quality Control and Life Cycle in the Nervous System In Vivo in the Absence of PINK1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Miro's N-terminal GTPase domain is required for transport of mitochondria into axons and dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Nebula/DSCR1 upregulation delays neurodegeneration and protects against APP-induced axonal transport defects by restoring calcineurin and GSK-3β signaling.PLoS genetics · 2013Article
- Loss of syd-1 from R7 neurons disrupts two distinct phases of presynaptic development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Simple microfluidic devices for in vivo imaging of C. elegans, Drosophila and zebrafish.Journal of visualized experiments : JoVE · 2012Article
- The p150(Glued) CAP-Gly domain regulates initiation of retrograde transport at synaptic termini.Neuron · 2012Article
- Mitochondrial dysfunction and oxidative stress contribute to the pathogenesis of spinocerebellar ataxia type 12 (SCA12).The Journal of biological chemistry · 2011Article
- Drosophila Acyl-CoA synthetase long-chain family member 4 regulates axonal transport of synaptic vesicles and is required for synaptic development and transmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Drosophila Miro is required for both anterograde and retrograde axonal mitochondrial transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Characterization of axonal transport defects in Drosophila Huntingtin mutants.Journal of neurogeneticsArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The distribution of mitochondria is sensitive to physiological stresses and changes in metabolic demands. Consequently, it is important to carefully define the conditions facilitating live imaging of mitochondrial transport in dissected animal preparations. In this study, we examined Schneider's and the haemolymph-like solutions HL3 and HL6 for their suitability to image mitochondrial transport in motor axons of dissected Drosophila melanogaster larvae. Overall, mitochondrial transport kinetics in larval motor axons appeared similar among all three solutions. Unexpectedly, HL3 solution selectively increased the length of mitochondria in the context of the net-direction of transport. We also found that mitochondrial transport is sensitive to the extracellular Ca(2+) but not glutamate concentration. High concentrations of extracellular glutamate affected only the ratio between motile and stationary mitochondria. Our study offers a valuable overview of mitochondrial transport kinetics in larval motor axons of Drosophila under various conditions, guiding future studies genetically dissecting mechanisms of mitochondrial transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.