ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2008
Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Seasonal influenza in adults and children--diagnosis, treatment, chemoprophylaxis, and institutional outbreak management: clinical practice guidelines of the Infectious Diseases Society of America.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2009Guideline
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- Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+ cells including macrophages yet decrease of the staining intensity of KUL01+ cells using clodronate liposomes did not affect the viral genome loads in chickens.Veterinary immunology and immunopathology · 2018Article
- Thick Smear is a Good Substitute for the Thin Smear in Parasitological Confirmation of Canine Visceral Leishmaniasis.The American journal of tropical medicine and hygiene · 2016Article
- Influenza Induced Cardiomyopathy: An Unusual Cause of Hypoxemia.Case reports in cardiology · 2015Article
- A highly sensitive europium nanoparticle-based immunoassay for detection of influenza A/B virus antigen in clinical specimens.Journal of clinical microbiology · 2014Article
- Factors influencing the diagnostic accuracy of the rapid influenza antigen detection test (RIADT): a cross-sectional study.BMJ open · 2014Article
- Recent advances in the diagnosis and treatment of influenza pneumonia.Current infectious disease reports · 2012Article
- Clinical validation of a point-of-care multiplexed in vitro immunoassay using monoclonal antibodies (the MSD influenza test) in four hospitals in Vietnam.Journal of clinical microbiology · 2012Article
- Delay in diagnosis of influenza virus in an elderly hospitalized patient: a fatal outcome.Clinical medicine insights. Case reports · 2012Article
- Impact of PCR for respiratory viruses on antibiotic use: theory and practice.Pediatric pulmonology · 2011Article
- Influenza-associated mortality among children - United States: 2007-2008.Influenza and other respiratory viruses · 2011Article
- Influenza vaccine given to pregnant women reduces hospitalization due to influenza in their infants.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2010Article
- The role of rapid antigen testing for influenza in the era of molecular diagnostics.Molecular diagnosis & therapy · 2010Review
- Serum 25-hydroxyvitamin d and the incidence of acute viral respiratory tract infections in healthy adults.PloS one · 2010Article
- Cytospin-enhanced immunofluorescence and impact of sample quality on detection of novel swine origin (H1N1) influenza virus.Journal of clinical microbiology · 2010Article
- Poor clinical sensitivity of rapid antigen test for influenza A pandemic (H1N1) 2009 virus.Emerging infectious diseases · 2009Article
- Gene expression signatures diagnose influenza and other symptomatic respiratory viral infections in humans.Cell host & microbe · 2009Article
- Establishing a surveillance network for severe lower respiratory tract infections in Korean infants and young children.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRapid tests have had a significant impact on influenza diagnosis, but more accurate tests are needed for hospitalized patients who test negative by rapid methods.
objectiveWe sought to determine the increased yield obtained from influenza RT-PCR in hospitalized patients compared to two rapid methods. STUDY
designBinax NOW, cytospin-enhanced direct immunofluoroescence (DFA), and influenza A and B multiplex TaqMan RT-PCR were performed on 237 clinical samples.
resultsBinax NOW detected 70 (53.0%), cytospin-DFA detected 127 (96.2%), and TaqMan RT-PCR detected 132 (100%) influenza-positive samples. The difference in sensitivity was significant between RT-PCR and Binax NOW (p<0.0001), but not between RT-PCR and cytospin-DFA (p=0.0736). Two samples testing positive for influenza B by all three methods, tested falsely positive for influenza A by Binax. Eight true positive samples did not become reactive by Binax until 30 min, and thus were counted as negative.
conclusionsThe accuracy of real-time RT-PCR should greatly improve the diagnosis of influenza in hospitals using simple rapid flu tests, but may have a more modest impact in hospitals with expertise in cytospin-DFA. Further studies are needed to determine the effect of influenza RT-PCR on patient management and costs in hospitalized patients.
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