ArticleTissue engineering. Part A2009
Interstitial fluid flow intensity modulates endothelial sprouting in restricted Src-activated cell clusters during capillary morphogenesis.
Article in Tissue engineering. Part A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 67 citations in OpenAlex.
- Development of a Synthetic Hydrogel to Foster Microvascularization of an Endometriosis Microphysiological System.Advanced healthcare materials · 2026Article
- Fluid flow to mimic organ function in 3DAPL bioengineering · 2023Review
- Dynamic Stimulations with Bioengineered Extracellular Matrix-Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Vascularized Tissue Organoids.Bioengineering (Basel, Switzerland) · 2023Review
- Mechanical regulation of the early stages of angiogenesis.Journal of the Royal Society, Interface · 2022Review
- Interstitial flow promotes the formation of functional microvascular networksAdvanced functional materials · 2022Article
- Aging-related structural change in 3D extracellular matrix affects its mechanics.Medical engineering & physics · 2022Article
- Design of an Integrated Microvascularized Human Skin-on-a-Chip Tissue Equivalent Model.Frontiers in bioengineering and biotechnology · 2022Article
- Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology.Communications biology · 2021Review
- Microfluidics for the study of mechanotransduction.Journal of physics D: Applied physics · 2020Article
- Balance of interstitial flow magnitude and vascular endothelial growth factor concentration modulates three-dimensional microvascular network formation.APL bioengineering · 2019Article
- Multi-flow channel bioreactor enables real-time monitoring of cellular dynamics in 3D engineered tissue.Communications biology · 2019Article
- Piezo1 mediates angiogenesis through activation of MT1-MMP signaling.American journal of physiology. Cell physiology · 2019Article
- Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds.Journal of visualized experiments : JoVE · 2018Article
- A cardiac patch from aligned microvessel and cardiomyocyte patches.Journal of tissue engineering and regenerative medicine · 2018Article
- Building branched tissue structures: from single cell guidance to coordinated construction.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Flow-induced HDAC1 phosphorylation and nuclear export in angiogenic sprouting.Scientific reports · 2016Article
- Traction Forces of Endothelial Cells under Slow Shear Flow.Biophysical journal · 2015Article
- Bioengineering Models for Breast Cancer Research.Breast cancer : basic and clinical research · 2015Review
- Fluid shear stress threshold regulates angiogenic sprouting.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Development of tissues in vitro with dimensions larger than 150 to 200 microm requires the presence of a functional vascular network. Therefore, we have studied capillary morphogenesis under controlled biological and biophysical conditions with the aim of promoting vascular structures in tissue constructs. We and others have previously demonstrated that physiological values of interstitial fluid flow normal to an endothelial monolayer in combination with vascular endothelial growth factor play a critical role during capillary morphogenesis by promoting cell sprouting. In the present work, we studied the effect that a range of interstitial flow velocities (0-50 microm/min) has in promoting the amount, length, and branching of developing sprouts during capillary morphogenesis. The number of capillary-like structures developed from human umbilical vein endothelial cell monolayers across the interstitial flow values tested was not significantly affected. Instead, the length and branching degree of the sprouts presented a significant maximum at flow velocities of 10 to 20 microm/min. More-over, at these same flow values, the phosphorylation level of Src also showed its peak. We discovered that capillary morphogenesis is restricted to patches of Src-activated cells (phosphorylated Src (pSrc)) at the monolayer, suggesting that the transduction pathway in charge of sensing the mechanical stimulus induced by flow is promoting predetermined mechanically sensitive areas (pSrc) to undergo capillary morphogenesis
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.