ArticleThe EMBO journal2008
Extracellular Alix regulates integrin-mediated cell adhesions and extracellular matrix assembly.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- 3D bioprinting patient-specific grafts for tendon/ligament repair in motion: emerging trends and challenges.Frontiers in bioengineering and biotechnology · 2025Review
- RNA packaging into extracellular vesicles: An orchestra of RNA-binding proteins?Journal of extracellular vesicles · 2020Review
- Small extracellular vesicles convey the stress-induced adaptive responses of melanoma cells.Scientific reports · 2019Article
- The Impact of the CD9 Tetraspanin on Lentivirus Infectivity and Exosome Secretion.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Alix is required during development for normal growth of the mouse brain.Scientific reports · 2017Article
- ALG-2 interacting protein-X (Alix) is essential for clathrin-independent endocytosis and signaling.Scientific reports · 2016Article
- ALG-2 activates the MVB sorting function of ALIX through relieving its intramolecular interaction.Cell discovery · 2015Article
- Golgi-situated endoplasmic reticulum α-1, 2-mannosidase contributes to the retrieval of ERAD substrates through a direct interaction with γ-COP.Molecular biology of the cell · 2013Article
- Protein interactions of the transcription factor Hoxa1.BMC developmental biology · 2012Article
- EhADH112 is a Bro1 domain-containing protein involved in the Entamoeba histolytica multivesicular bodies pathway.Journal of biomedicine & biotechnology · 2012Article
- The Phe105 loop of Alix Bro1 domain plays a key role in HIV-1 release.Structure (London, England : 1993) · 2011Article
- A proteomic and cellular analysis of uropods in the pathogen Entamoeba histolytica.PLoS neglected tropical diseases · 2011Article
- Decoding the intrinsic mechanism that prohibits ALIX interaction with ESCRT and viral proteins.The Biochemical journal · 2010Article
- Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.BMC structural biology · 2010Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Alix (ALG-2-interacting protein X), a cytoplasmic adaptor protein involved in endosomal sorting and actin cytoskeleton assembly, is required for the maintenance of fibroblast morphology. As Alix has sequence similarity to adhesin in Entamoeba histolytica, and we observed that Alix is secreted, we determined whether extracellular Alix affects fibroblast morphology. Here, we demonstrate that secreted Alix is deposited on the substratum of non-immortalized WI38 fibroblasts. Antibody binding to extracellular Alix retards WI38 cell adhesion and spreading on fibronectin and vitronectin. Alix knockdown in WI38 cells reduces spreading and fibronectin assembly, and the effect is partially complemented by coating recombinant Alix on the cell substratum. Immortalized NIH/3T3 fibroblasts deposit less Alix on the substratum and have defects in alpha5beta1-integrin functions. Coating recombinant Alix on the culture substratum for NIH/3T3 cells promotes alpha5beta1-integrin-mediated cell adhesions and fibronectin assembly, and these effects require the aa 605-709 region of Alix. These findings demonstrate that a sub-population of Alix localizes extracellularly and regulates integrin-mediated cell adhesions and fibronectin matrix assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.