ArticleJournal of applied physiology (Bethesda, Md. : 1985)2008
GFP-expressing locus ceruleus neurons from Prp57 transgenic mice exhibit CO2/H+ responses in primary cell culture.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Minocycline attenuates panicogenic responses in a COTranslational psychiatry · 2026Trial
- Criteria for central respiratory chemoreceptors: experimental evidence supporting current candidate cell groups.Frontiers in physiology · 2023Review
- Enhanced firing of locus coeruleus neurons and SK channel dysfunction are conserved in distinct models of prodromal Parkinson's disease.Scientific reports · 2022Article
- Locus Coeruleus Acid-Sensing Ion Channels Modulate Sleep-Wakefulness and State Transition from NREM to REM Sleep in the Rat.Neuroscience bulletin · 2021Article
- Critical Role of Oxidatively Damaged DNA in Selective Noradrenergic Vulnerability.Neuroscience · 2019Article
- Brainstem catecholaminergic neurones and breathing control during postnatal development in male and female rats.The Journal of physiology · 2018Article
- Medullary 5-HT neurons: Switch from tonic respiratory drive to chemoreception during postnatal development.Neuroscience · 2017Article
- A HCO(3)(-)-dependent mechanism involving soluble adenylyl cyclase for the activation of Ca²⁺ currents in locus coeruleus neurons.Biochimica et biophysica acta · 2014Article
- GABAergic synaptic inputs of locus coeruleus neurons in wild-type and Mecp2-null mice.American journal of physiology. Cell physiology · 2013Article
- Central chemoreceptors: locations and functions.Comprehensive Physiology · 2012Review
- The disruption of central CO2 chemosensitivity in a mouse model of Rett syndrome.American journal of physiology. Cell physiology · 2011Article
- Involvement of TRP channels in the CO₂ chemosensitivity of locus coeruleus neurons.Journal of neurophysiology · 2011Article
- Genetic inactivation of Kcnj16 identifies Kir5.1 as an important determinant of neuronal PCO2/pH sensitivity.The Journal of biological chemistry · 2011Article
- Julius H. Comroe, Jr., distinguished lecture: central chemoreception: then ... and now.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Article
- The locus coeruleus and central chemosensitivity.Respiratory physiology & neurobiology · 2010Review
- Medullary serotonin neurons and their roles in central respiratory chemoreception.Respiratory physiology & neurobiology · 2010Review
- Central respiratory chemoreception.The Journal of comparative neurology · 2010Review
- Central chemoreception in wakefulness and sleep: evidence for a distributed network and a role for orexin.Journal of applied physiology (Bethesda, Md. : 1985) · 2010Review
- Serotonergic mechanisms on breathing modulation in the rat locus coeruleus.Pflugers Archiv : European journal of physiology · 2010Article
- Pathophysiology of locus ceruleus neurons in a mouse model of Rett syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The locus ceruleus (LC) contains neurons that increase their firing rate (FR) in vitro when exposed to elevated CO(2)/H(+) and have been proposed to influence the respiratory network to make compensatory adjustments in ventilation. Prp57 transgenic mice express green fluorescent protein (GFP) in the LC and were used to isolate, culture, and target LC neurons for electrophysiological recording. We hypothesized that GFP-LC neurons would exhibit CO(2)/H(+) chemosensitivity under primary culture conditions, evidenced as a change in FR. This is the first study to quantify CO(2)/H(+) responses in LC neuron FR in cell culture. Neurons were continuously bathed with solutions containing antagonists of glutamate and GABA receptors, and the acid-base status was changed from control (5% CO(2); pH approximately 7.4) to hypercapnic acidosis (9% CO(2); pH approximately 7.2) and hypocapnic alkalosis (3% CO(2); pH approximately 7.6). FR was quantified during perforated patch current clamp recordings. Approximately 86% of GFP-LC neurons were stimulated, and approximately 14% were insensitive to changes in CO(2)/H(+). The magnitude of the response of these neurons depended on the baseline FR, ranging from 155.9 +/- 6% when FR started at 2.95 +/- 0.49 Hz to 381 +/- 55.6% when FR started at 1.32 +/- 0.31 Hz. These results demonstrate that cultured LC neurons from Prp57 transgenic mice retain functional sensing molecules necessary for CO(2)/H(+) responses. Prp57 transgenic mice will serve as a valuable model to delineate mechanisms involved in CO(2)/H(+) responsiveness in catecholaminergic neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.