ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2008
T-cadherin modulates hepatocyte functions in vitro.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
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- Decellularized Liver Nanofibers Enhance and Stabilize the Long-Term Functions of Primary Human Hepatocytes In Vitro.Advanced healthcare materials · 2023Article
- Introducing CELLBLOKSIn vitro models · 2022Article
- Latest impact of engineered human liver platforms on drug development.APL bioengineering · 2021Review
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- Controlled Apoptosis of Stromal Cells to Engineer Human Microlivers.Advanced functional materials · 2020Article
- Assessing the compatibility of primary human hepatocyte culture within porous silk sponges.RSC advances · 2020Article
- Intermittent Starvation Extends the Functional Lifetime of Primary Human Hepatocyte Cultures.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Validation of miR-20a as a Tumor Suppressor Gene in Liver Carcinoma Using Hepatocyte-Specific Hyperactive piggyBac Transposons.Molecular therapy. Nucleic acids · 2020Article
- Emerging trends in modeling human liver diseaseAPL bioengineering · 2019Article
- Long-Term Engineered Cultures of Primary Mouse Hepatocytes for Strain and Species Comparison Studies During Drug Development.Gene expression · 2019Article
- Advances in Engineered Human Liver Platforms for Drug Metabolism Studies.Drug metabolism and disposition: the biological fate of chemicals · 2018Article
- Microfabrication of liver and heart tissues for drug development.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- A polyelectrolyte multilayer platform for investigating growth factor delivery modes in human liver cultures.Journal of biomedical materials research. Part A · 2018Article
- Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies.Cellular and molecular gastroenterology and hepatology · 2018Review
- A Cell Culture Platform to Maintain Long-term Phenotype of Primary Human Hepatocytes and Endothelial Cells.Cellular and molecular gastroenterology and hepatology · 2018Article
- Pluripotent Stem Cell-Derived Human Tissue: Platforms to Evaluate Drug Metabolism and Safety.The AAPS journal · 2017Review
- Micropatterned Co-Cultures of Human Hepatocytes and Stromal Cells for the Assessment of Drug Clearance and Drug-Drug Interactions.Current protocols in toxicology · 2017Article
- High-Throughput Platform for Identifying Molecular Factors Involved in Phenotypic Stabilization of Primary Human Hepatocytes In Vitro.Journal of biomolecular screening · 2016Article
- Micropatterned coculture of primary human hepatocytes and supportive cells for the study of hepatotropic pathogens.Nature protocols · 2015Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Primary hepatocytes from several different species rapidly lose viability and phenotypic functions on isolation from their native microenvironment of the liver. Stromal cells derived from both within and outside the liver can induce phenotypic functions in primary hepatocytes in vitro; however, the molecular mediators underlying this "coculture effect" have not been fully elucidated. We have previously developed a functional genomic screen utilizing cocultures of hepatocytes and 3T3 fibroblasts to identify such candidate hepatocyte-function-inducing molecules. In particular, truncated-cadherin (T-cadherin) was identified as a potential molecule of interest in induction of hepatic functions. Here we demonstrate that liver-specific functions of primary rat hepatocytes are induced on cocultivation with Chinese hamster ovary cells engineered to express T-cadherin on their surface as compared with wild-type controls. Additionally, culture of cells on substrata presenting recombinant T-cadherin protein (acellular presentation) enhanced hepatic functions in both pure hepatocyte cultures and in hepatocyte-stromal cocultures lacking endogenous T-cadherin expression. Collectively, these data indicate that both cellular and acellular presentation of T-cadherin can be used to modulate the hepatocyte phenotype in vitro for tissue engineering applications. Our work suggests potential avenues for investigating the role of T-cadherin on hepatocellular function in vivo in settings such as embryogenesis and liver pathology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.