ArticleAnalytical biochemistry2008
Enzymatic analysis of uridine diphosphate N-acetyl-D-glucosamine.
Article in Analytical biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system.Acta crystallographica. Section D, Structural biology · 2025Article
- mRNA caps accumulate in stress granules and are essential for their formation.Cellular and molecular life sciences : CMLS · 2025Article
- Characterization of a novel glycocin from a thermophile.bioRxiv : the preprint server for biology · 2025Article
- Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor.ACS sensors · 2023Article
- Enzymatic assay for UDP-GlcNAc and its application in the parallel assessment of substrate availability and protein O-GlcNAcylation.Cell reports methods · 2023Article
- Enzyme-based assay for quantification of UDP-GlcNAc in cells and tissues.Cell reports methods · 2023Article
- A kinetic rationale for functional redundancy in fatty acid biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA.Nucleic acids research · 2019Article
- A structural and functional analysis of the glycosyltransferase BshA from Staphylococcus aureus: Insights into the reaction mechanism and regulation of bacillithiol production.Protein science : a publication of the Protein Society · 2019Article
- Deficiency of AtGFAT1 activity impairs growth, pollen germination and tolerance to tunicamycin in Arabidopsis.Journal of experimental botany · 2019Article
- Functional Characterization of the ycjQRS Gene Cluster from Escherichia coli: A Novel Pathway for the Transformation of d-Gulosides to d-Glucosides.Biochemistry · 2019Article
- Toxin ζ Reduces the ATP and Modulates the Uridine Diphosphate-N-acetylglucosamine Pool.Toxins · 2019Article
- Construction and characterization of a Saccharomyces cerevisiae strain able to grow on glucosamine as sole carbon and nitrogen source.Scientific reports · 2018Article
- The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.Nature communications · 2018Article
- YvcK, a protein required for cell wall integrity and optimal carbon source utilization, binds uridine diphosphate-sugars.Scientific reports · 2017Article
- Evidence that biosynthesis of the second and third sugars of the archaellin Tetrasaccharide in the archaeon Methanococcus maripaludis occurs by the same pathway used by Pseudomonas aeruginosa to make a di-N-acetylated sugar.Journal of bacteriology · 2015Article
- N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.Microbiology and molecular biology reviews : MMBR · 2014Review
- Identification of genes involved in the biosynthesis of the third and fourth sugars of the Methanococcus maripaludis archaellin N-linked tetrasaccharide.Journal of bacteriology · 2013Article
- Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction.The EMBO journal · 2013Article
- ε/ζ systems: their role in resistance, virulence, and their potential for antibiotic development.Journal of molecular medicine (Berlin, Germany) · 2011Review
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Authors and funding
2 authors.
Funding
Abstract
The Methanococcus maripaludis MMP0352 protein belongs to an oxidoreductase family that has been proposed to catalyze the NAD(+)-dependent oxidation of the 3'' position of uridine diphosphate N-acetyl-D-glucosamine (UDP-GlcNAc), forming a 3-hexulose sugar nucleotide. The heterologously expressed MMP0352 protein was purified and shown to efficiently catalyze UDP-GlcNAc oxidation, forming one NADH equivalent. This enzyme was used to develop a fixed endpoint fluorometric method to analyze UDP-GlcNAc. The enzyme is highly specific for this acetamido sugar nucleotide, and the procedure had a detection limit of 0.2 microM UDP-GlcNAc in a 1-ml sample. Using the method of standard addition, UDP-GlcNAc concentrations were measured in deproteinized extracts of Escherichia coli, Saccharomyces cerevisiae, and HeLa carcinoma cells. Equivalent concentrations were determined by both enzymatic and chromatographic analyses, validating this method. This procedure can be adapted for the high-throughput analysis of changes in cellular UDP-GlcNAc concentrations in time series experiments or inhibitor screens.
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