ArticleJournal of industrial microbiology & biotechnology2008
A comparison of methods for total community DNA preservation and extraction from various thermal environments.
Article in Journal of industrial microbiology & biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Effects of heat-assisted sample desiccation on microbiome surveys.Environmental microbiome · 2026Article
- Microbially derived essential amino acids compensate for dietary deficiencies in an ecologically relevant mammalian host.The ISME journal · 2026Article
- Unearthing the genetic resources of Arabian sea seamount and metagenomic insights into phosphate cycling genes for next generation plant biostimulants.Scientific reports · 2025Article
- Effects of Vacuum-Heat-Assisted Sample Desiccation on Microbiome Surveys.Molecular ecology resources · 2025Article
- Secretory IgM regulates gut microbiota homeostasis and metabolism.Nature microbiology · 2025Article
- The telencephalon is a neuronal substrate for systemic inflammatory responses in teleosts via polyamine metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A brain microbiome in salmonids at homeostasis.Science advances · 2024Article
- Sex-dependent effects of mechanical delousing on the skin microbiome of broodstock Atlantic salmon (Salmo salar L.).Scientific reports · 2023Article
- Limits to the three domains of life: lessons from community assembly along an Antarctic salinity gradient.Extremophiles : life under extreme conditions · 2022Article
- Capturing marine microbiomes and environmental DNA: A field sampling guide.Frontiers in microbiology · 2022Review
- The lungfish cocoon is a living tissue with antimicrobial functions.Science advances · 2021Article
- Prediction of Genes That Function in Methanogenesis and COMicroorganisms · 2021Article
- In silico determination of nitrogen metabolism in microbes from extreme conditions using metagenomics.Archives of microbiology · 2021Article
- Lithogenic hydrogen supports microbial primary production in subglacial and proglacial environments.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar).PloS one · 2021Article
- Got Glycogen?: Development and Multispecies Validation of the Novel Preserve, Precipitate, Lyse, Precipitate, Purify (PPLPP) Workflow for Environmental DNA Extraction from Longmire's Preserved Water Samples.Journal of biomolecular techniques : JBT · 2020Article
- Isotopic and genetic methods reveal the role of the gut microbiome in mammalian host essential amino acid metabolism.Proceedings. Biological sciences · 2020Article
- Specialization of mucosal immunoglobulins in pathogen control and microbiota homeostasis occurred early in vertebrate evolution.Science immunology · 2020Article
- Robust sampling and preservation of DNA for microbial community profiling in field experiments.BMC research notes · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The widespread use of molecular techniques in studying microbial communities has greatly enhanced our understanding of microbial diversity and function in the natural environment and contributed to an explosion of novel commercially viable enzymes. One of the most promising environments for detecting novel processes, enzymes, and microbial diversity is hot springs. We examined potential biases introduced by DNA preservation and extraction methods by comparing the quality, quantity, and diversity of environmental DNA samples preserved and extracted by commonly used methods. We included samples from sites representing the spectrum of environmental conditions that are found in Yellowstone National Park thermal features. Samples preserved in a non-toxic sucrose lysis buffer (SLB), along with a variation of a standard DNA extraction method using CTAB resulted in higher quality and quantity DNA than the other preservation and extraction methods tested here. Richness determined using DGGE revealed that there was some variation within replicates of a sample, but no statistical difference among the methods. However, the sucrose lysis buffer preserved samples extracted by the CTAB method were 15-43% more diverse than the other treatments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.