ArticleClinical and vaccine immunology : CVI2008
Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Investigation of Comorbidity and Risk Factors Analysis During Lumpy Skin Disease Outbreaks in India.Microorganisms · 2025Article
- CRISPR-Cas-Based Pen-Side Diagnostic Tests forMicroorganisms · 2024Article
- Article
- Research on Integrated Management for Cattle Fever Ticks and Bovine Babesiosis in the United States and Mexico: Current Status and Opportunities for Binational Coordination.Pathogens (Basel, Switzerland) · 2020Review
- Bioactive Compounds, Pharmacological Actions, and Pharmacokinetics of Wormwood (Antibiotics (Basel, Switzerland) · 2020Review
- Diagnostic Tools for the Identification ofPathogens (Basel, Switzerland) · 2019Review
- Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt.Parasites & vectors · 2015Article
- Validation and field evaluation of a competitive enzyme-linked immunosorbent assay for diagnosis of Babesia bovis infections in Argentina.Clinical and vaccine immunology : CVI · 2012Article
- Current advances in detection and treatment of babesiosis.Current medicinal chemistry · 2012Review
- One Health approach to identify research needs in bovine and human babesioses: workshop report.Parasites & vectors · 2010Article
- Seroprevalence estimation and management factors associated with high herd seropositivity for Babesia bovis in commercial dairy farms of Puerto Rico.Tropical animal health and production · 2009Article
- Evaluation of Babesia bigemina 200 kDa recombinant antigen in enzyme-linked immunosorbent assay.Parasitology research · 2009Article
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15 authors.
Funding
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Abstract
A competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigemina rhoptry-associated protein 1a was validated for international use. Receiver operating characteristic analysis revealed 16% inhibition as the threshold for a negative result, with an associated specificity of 98.3% and sensitivity of 94.7%. Increasing the threshold to 21% increased the specificity to 100% but modestly decreased the sensitivity to 87.2%. By using 21% inhibition, the positive predictive values ranged from 90.7% (10% prevalence) to 100% (95% prevalence) and the negative predictive values ranged from 97.0% (10% prevalence) to 48.2% (95% prevalence). The assay was able to detect serum antibody as early as 7 days after intravenous inoculation. The cELISA was distributed to five different laboratories along with a reference set of 100 defined bovine serum samples, including known positive, known negative, and field samples. The pairwise concordance among the five laboratories ranged from 100% to 97%, and all kappa values were above 0.8, indicating a high degree of reliability. Overall, the cELISA appears to have the attributes necessary for international application.
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