ArticleJournal of clinical microbiology2008
Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 95 citations in OpenAlex.
- Molecular Identification and Phylogenetic Characterization of Yeast Species Isolated from Complete Denture in Sulaymaniyah, Iraq.Polish journal of microbiology · 2025Article
- Diagnosis and management of invasive fungal infections due to non-Aspergillus moulds.The Journal of antimicrobial chemotherapy · 2025Review
- Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.Journal of fungi (Basel, Switzerland) · 2024Review
- Performance of a Real-Time PCR Assay for the Detection of FiveJournal of fungi (Basel, Switzerland) · 2023Article
- Pulmonary Cryptococcosis.Journal of fungi (Basel, Switzerland) · 2022Review
- A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Development of a Real-Time PCR Assay to Identify and Distinguish betweenJournal of fungi (Basel, Switzerland) · 2022Article
- A Real Time PCR strategy for the detection and quantification of Candida albicans in human blood.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Multiplexed-tandem PCR (MT-PCR) assay to detect and differentiate gastrointestinal nematodes of alpacas.Parasites & vectors · 2018Article
- Identification and Characterization of Fusarium proliferatum, a New Species of Fungi that Cause Fungal Keratitis.Scientific reports · 2018Article
- Performance of a Quantitative PCR-Based Assay and Beta-d-Glucan Detection for Diagnosis of Invasive Candidiasis in Very-Low-Birth-Weight Preterm Neonatal Patients (CANDINEO Study).Journal of clinical microbiology · 2017Observational
- A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification.Journal of applied genetics · 2017Article
- Candida colonization as a risk marker for invasive candidiasis in mixed medical-surgical intensive care units: development and evaluation of a simple, standard protocol.Journal of clinical microbiology · 2015Article
- Rapid identification of medically important Candida isolates using high resolution melting analysis.PloS one · 2015Article
- Semiquantitative multiplexed tandem PCR for detection and differentiation of four Theileria orientalis genotypes in cattle.Journal of clinical microbiology · 2015Article
- A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens.Biomolecular detection and quantification · 2014Review
- A multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patients.BMC infectious diseases · 2014Article
- A quick and low-cost PCR-based assay for Candida spp. identification in positive blood culture bottles.BMC infectious diseases · 2013Article
- Saponin promotes rapid identification and antimicrobial susceptibility profiling of Gram-positive and Gram-negative bacteria in blood cultures with the Vitek 2 system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Polymerase chain reaction-based assays for the diagnosis of invasive fungal infections.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the first development and evaluation of a rapid multiplex tandem PCR (MT-PCR) assay for the detection and identification of fungi directly from blood culture specimens that have been flagged as positive. The assay uses a short-cycle multiplex amplification, followed by 12 simultaneous PCRs which target the fungal internal transcribed spacer 1 (ITS1) and ITS2 region, elongation factor 1-alpha (EF1-alpha), and beta-tubulin genes to identify 11 fungal pathogens: Candida albicans, Candida dubliniensis, Candida glabrata, Candida guilliermondii, Candida krusei, Candida parapsilosis complex, Candida tropicalis, Cryptococcus neoformans complex, Fusarium solani, Fusarium species, and Scedosporium prolificans. The presence or absence of a fungal target was confirmed by melting curve analysis. Identification by MT-PCR correlated with culture-based identification for 44 (100%) patients. No cross-reactivity was detected in 200 blood culture specimens that contained bacteria or in 30 blood cultures without microorganisms. Fungi were correctly identified in five specimens with bacterial coinfection and in blood culture samples that were seeded with a mixture of yeast cells. The MT-PCR assay was able to provide rapid (<2 h), sensitive, and specific simultaneous detection and identification of fungal pathogens directly from blood culture specimens.
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