ArticleThe Journal of antimicrobial chemotherapy2008
Functional characterization of Tn1331 gene cassettes.
Article in The Journal of antimicrobial chemotherapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons.Scientific reports · 2026Article
- Aminoglycoside 6'-Antibiotics (Basel, Switzerland) · 2020Article
- Carbapenemases: TransformingBiomolecules · 2020Review
- Assessing genetic diversity and similarity of 435 KPC-carrying plasmids.Scientific reports · 2019Article
- SmallFrontiers in microbiology · 2019Article
- Amikacin: Uses, Resistance, and Prospects for Inhibition.Molecules (Basel, Switzerland) · 2017Review
- Characterization of Tn6238 with a new allele of aac(6')-Ib-cr.Antimicrobial agents and chemotherapy · 2015Article
- Polycistronic transcription of fused cassettes and identification of translation initiation signals in an unusual gene cassette array from Pseudomonas aeruginosa.F1000Research · 2013Article
- Rise and dissemination of aminoglycoside resistance: the aac(6')-Ib paradigm.Frontiers in microbiology · 2013Article
- Achromobacter xylosoxidans: an emerging pathogen carrying different elements involved in horizontal genetic transfer.Current microbiology · 2012Article
- Aminoglycoside modifying enzymes.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2010Review
- Novel integron gene cassette arrays identified in a global collection of multi-drug resistant non-typhoidal Salmonella enterica.Current microbiology · 2010Article
- A blaVEB-1 variant, blaVEB-6, associated with repeated elements in a complex genetic structure.Antimicrobial agents and chemotherapy · 2009Article
- Plasmid-Mediated Antibiotic Resistance and Virulence in Gram-negatives: theMicrobiology spectrumArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
objectivesThe transposon Tn1331 possesses a region including three antibiotic resistance genes with the structure aac(6')-Ib-attC-aadA1-attI1*-bla(OXA-9)-attC, which potentially includes four gene cassettes. Experimental data on the mobility of fusion cassettes as well as those on mobility of cassettes in a genetic environment such as Tn1331, which lacks an integrase gene, are limited. Therefore, experiments using pJHCMW1, a plasmid harbouring this transposon, in the presence of IntI1 supplied in trans were carried out to define which cassettes are mobile in vivo.
methodsIn vivo excision of resistance genes was investigated in Escherichia coli cells harbouring pJHCMW1 and in a recombinant clone that included the intI1 gene under the control of the P(tac) promoter. Plasmid DNA was purified and subjected to PCR analysis, and DNA sequencing of PCR products was performed to determine whether excision had occurred. RESULTS AND
conclusionsIn vivo recombination experiments showed that the fused aadA1-attI1*-bla(OXA-9)-attC gene cassette was excised in the presence of IntI1. The excision of a DNA fragment including aadA1-attI1* was also detected but at a lower frequency. The analysis of the latter recombination reaction showed that, although attI1* includes only a small fraction of the complete attI1 sequence, it is still used as a substrate by IntI1, albeit in a very inefficient manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.