ArticleNucleic acids research2008
Features of 80S mammalian ribosome and its subunits.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Tetracyclines, a timely resurgence or doomed to fail again?Journal of medical microbiology · 2026Review
- Observing intersubunit dynamics in single yeast ribosomes.Nucleic acids research · 2026Article
- Comprehensive mutational analysis of the sequence-function relationship within a viral internal ribosome entry site.Nucleic acids research · 2025Article
- Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline.Nature communications · 2024Article
- Mechanistic Approaches to the Application of Nano-Zinc in the Poultry and Biomedical Industries: A Comprehensive Review of Future Perspectives and Challenges.Molecules (Basel, Switzerland) · 2023Review
- Contribution of miRNAs, tRNAs and tRFs to Aberrant Signaling and Translation Deregulation in Lung Cancer.Cancers · 2020Article
- Tetracyclines Modify Translation by Targeting Key Human rRNA Substructures.Cell chemical biology · 2018Article
- Nucleolus as an emerging hub in maintenance of genome stability and cancer pathogenesis.Oncogene · 2018Review
- Article
- Perturbation of RNA Polymerase I transcription machinery by ablation of HEATR1 triggers the RPL5/RPL11-MDM2-p53 ribosome biogenesis stress checkpoint pathway in human cells.Cell cycle (Georgetown, Tex.) · 2018Article
- Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.Nature communications · 2016Article
- What Is the Impact of mRNA 5' TL Heterogeneity on Translational Start Site Selection and the Mammalian Cellular Phenotype?Frontiers in genetics · 2016Review
- Functional Dynamics within the Human Ribosome Regulate the Rate of Active Protein Synthesis.Molecular cell · 2015Article
- Interaction of tRNA with eukaryotic ribosome.International journal of molecular sciences · 2015Review
- Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.Nature structural & molecular biology · 2014Article
- Article
- The translation of translational control by FMRP: therapeutic targets for FXS.Nature neuroscience · 2013Review
- Translocation at work.Nature structural & molecular biology · 2013Article
- Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Selenocysteine insertion sequence (SECIS)-binding protein 2 alters conformational dynamics of residues involved in tRNA accommodation in 80 S ribosomes.The Journal of biological chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is generally believed that basic features of ribosomal functions are universally valid, but a systematic test still stands out for higher eukaryotic 80S ribosomes. Here we report: (i) differences in tRNA and mRNA binding capabilities of eukaryotic and bacterial ribosomes and their subunits. Eukaryotic 40S subunits bind mRNA exclusively in the presence of cognate tRNA, whereas bacterial 30S do bind mRNA already in the absence of tRNA. 80S ribosomes bind mRNA efficiently in the absence of tRNA. In contrast, bacterial 70S interact with mRNA more productively in the presence rather than in the absence of tRNA. (ii) States of initiation (P(i)), pre-translocation (PRE) and post-translocation (POST) of the ribosome were checked and no significant functional differences to the prokaryotic counterpart were observed including the reciprocal linkage between A and E sites. (iii) Eukaryotic ribosomes bind tetracycline with an affinity 15 times lower than that of bacterial ribosomes (K(d) 30 microM and 1-2 microM, respectively). The drug does not effect enzymatic A-site occupation of 80S ribosomes in contrast to non-enzymatic tRNA binding to the A-site. Both observations explain the relative resistance of eukaryotic ribosomes to this antibiotic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.