ArticleNucleic acids research2008
Intronically encoded siRNAs improve dynamic range of mammalian gene regulation systems and toggle switch.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Modular Scalable Synthetic Gene Circuits for Complex Functions Within Minimal Computational Layers in Human Cells.Nature communications · 2026Article
- A platform for post-translational spatiotemporal control of cellular proteins.Synthetic biology (Oxford, England) · 2021Article
- Components from the Human c-myb Transcriptional Regulation System Reactivate Epigenetically Repressed Transgenes.International journal of molecular sciences · 2020Article
- A Synthetic Gene Circuit for Self-Regulating Delivery of Biologic Drugs in Engineered Tissues.Tissue engineering. Part A · 2019Article
- Designing cell function: assembly of synthetic gene circuits for cell biology applications.Nature reviews. Molecular cell biology · 2018Review
- Article
- Genome Engineering of Stem Cells for Autonomously Regulated, Closed-Loop Delivery of Biologic Drugs.Stem cell reports · 2017Article
- Mammalian synthetic biology for studying the cell.The Journal of cell biology · 2017Review
- Molecular recordings by directed CRISPR spacer acquisition.Science (New York, N.Y.) · 2016Article
- Mammalian synthetic biology: emerging medical applications.Journal of the Royal Society, Interface · 2015Review
- Permanent genetic memory with >1-byte capacity.Nature methods · 2014Article
- Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.Molecular cell · 2014Article
- Designable DNA-binding domains enable construction of logic circuits in mammalian cells.Nature chemical biology · 2014Article
- Linear population allocation by bistable switches in response to transient stimulation.PloS one · 2014Article
- Biomedically relevant circuit-design strategies in mammalian synthetic biology.Molecular systems biology · 2013Review
- Emerging biomedical applications of synthetic biology.Nature reviews. Genetics · 2011Review
- An engineered mammalian band-pass network.Nucleic acids research · 2010Article
- A synthetic low-frequency mammalian oscillator.Nucleic acids research · 2010Article
- Analysis of human small nucleolar RNAs (snoRNA) and the development of snoRNA modulator of gene expression vectors.Molecular biology of the cell · 2010Article
- Eukaryotic systems broaden the scope of synthetic biology.The Journal of cell biology · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Applications of conditional gene expression, whether for therapeutic or basic research purposes, are increasingly requiring mammalian gene control systems that exhibit far tighter control properties. While numerous approaches have been used to improve the widely used Tet-regulatory system, many applications, particularly with respect to the engineering of synthetic gene networks, will require a broader range of tightly performing gene control systems. Here, a generically applicable approach is described that utilizes intronically encoded siRNA on the relevant transregulator construct, and siRNA sequence-specific tags on the reporter construct, to minimize basal gene activity in the off-state of a range of common gene control systems. To demonstrate tight control of residual expression the approach was successfully used to conditionally express the toxic proteins RipDD and Linamarase. The intronic siRNA concept was also extended to create a new generation of compact, single-vector, autoinducible siRNA vectors. Finally, using improved regulation systems a mammalian epigenetic toggle switch was engineered that exhibited superior in vitro and in vivo induction characteristics in mice compared to the equivalent non-intronic system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.